烟草科技2019，Vol.52Issue(6)：1-8,8.
多腺毛烟草K326品种基因编辑材料的创制与分析
Creating a high trichome density variety, hairy K326, by CRISPR-Cas9 technique
摘要
Abstract
Tobacco trichomes are closely related to disease resistance and leaf quality. To improve tobacco trichome density and create a high trichome density variety, CRISPR-Cas9 technique was used to knock out NtCycB2, which negatively regulated trichome formation, in cv. K326. A high trichome density line was obtained as a result, named as HK326 (Hairy K326). Furthermore, the effects of NtCycB2 on the agronomic traits and chemical composition of flue-cured tobacco were studied. The results showed that HK326 grew and developed normally, and its agronomic trait was no different to the controls. The trichome density on the surface of HK326 leaves increased. Glandular trichomes of HK326 had strongly biosynthetic and secretory functions, and cembranoids content significantly raised. The total content of neutral aroma components in flue-cured leaves of HK326 was increased, especially solanone and neophytadiene. Therefore, the trichome density, growing, development and main aroma component accumulation of K326 was promoted by knocking out NtCycB2, which suggested that HK326 had promising application potential in tobacco variety improvement.关键词
烟草/K326/NtCycB2/基因编辑/腺毛/密度/西柏烷二萜Key words
Tobacco/K326/NtCycB2/Gene editing/Trichome/Density/Cembranoid diterpene分类
轻工纺织引用本文复制引用
PAN Yang,WANG Zhaojun,LI Xuejun,YAN Xiaoxiao,ZHANG Hongying,SUN Jiping,CUI Hong..多腺毛烟草K326品种基因编辑材料的创制与分析[J].烟草科技,2019,52(6):1-8,8.基金项目
河南省自然科学基金项目"MeJA诱导烟草分泌型腺毛发生关键调控基因的筛选和功能研究"(182300410094). （182300410094）
