烟草科技2019，Vol.52Issue(6)：23-29,7.
云南不同仓储地复烤烟叶表面醇化期间可培养真菌的动态变化
Population dynamics of culturable fungi on leaf surface of redried tobacco during aging in different areas of Yunnan Province
摘要
Abstract
To investigate the diversity of culturable fungi on the surface of redried tobacco leaves of K326 during aging in different areas of Yunnan, the morphological features and 18S rDNA sequences of the fungi were analyzed to study the community structure. The results showed that a total of 2 963 culturable fungi were obtained from 220 samples of redried tobacco leaves via separation and purification. With prolonged aging time, the quantity of culturable fungi on the surface of tobacco leaves from four areas presented a down-up-down-stable trend. By morphological features and 18S rDNA sequences, 39 genera (species) were identified. The community structure and variation of fungi differed on the surface of redried tobacco leaves according to different aging times and areas. However, the dominant genus was Rhizopus spp.关键词
K326/复烤烟叶/醇化/真菌/18S rDNA/种群结构Key words
K326/Redried tobacco leaf/Aging/Fungus/18S rDNA/Community structure分类
轻工纺织引用本文复制引用
BAI Weixiao,WU Guangli,WANG Yi,SONG Pengfei,MA Yongkai,WU Lijun,WEI Yunlin,JI Xiuling,LI Haiyan..云南不同仓储地复烤烟叶表面醇化期间可培养真菌的动态变化[J].烟草科技,2019,52(6):23-29,7.基金项目
国家自然科学基金地区科学基金项目"抗白粉病野生蔷薇内生真菌多样性及其抗病的分子机制"(31560566) （31560566）
云南中烟有限责任公司科技开发项目"微生物技术在促进烟叶醇化中的研究与应用"(2016YL03-1)和"高端优质烟叶提高烟叶醇化养护效率技术"(2016YL03-2). （2016YL03-1）
