烟草科技2019，Vol.52Issue(6)：35-41,7.
MAPK信号通路调节卷烟烟气诱导的炎症因子的释放
MAPK pathway-regulated cigarette smoke induced inflammatory cytokine release
XU Liangtao 1LI Xiang 1WANG Huiting 1LIU Kejian 1XIE Fuwei 1LIU Huimin 1XIE Jianping1
作者信息
- 1. Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China
- 折叠
摘要
Abstract
To evaluate the toxic effects of cigarette smoke on human bronchial epithelial cells (BEAS-2B cells), the viability of BEAS-2B cells was detected with a cell counting kit (CCK-8) and the cell morphology was observed under an optical microscopy. Western Blot assay was conducted to detect the expression variations of extracellular-regulated protein kinases 1/2 (ERK1/2) and c-Jun N-terminal kinase (JNK1/2) and the phosphorylation level of p38. The release levels of inflammatory cytokines interleukin-(IL-)1 β, IL-6 and IL-8 were detected by enzyme-linked immunosorbent assay. The results showed that: 1) The viability of BEAS-2B cells significantly decreased after exposure to cigarette smoke. 2) Exposure to cigarette smoke activated the expression of phospho-(P-)ERK1/2, P-JNK1/2, P-p38 pathways and induced the release of inflammatory cytokines IL-1 β, IL-6 and IL-8. 3) The release levels of inflammatory cytokines decreased significantly after ERK1/2, JNK1/2 and p38 pathways’being inhibited by specific inhibitors. In conclusion, the inflammation induced by cigarette smoke is regulated by mitogen-activated protein kinase (MAPK) pathways. These results provide a reference for the mechanism analysis of pulmonary inflammatory diseases induced by cigarette smoke.关键词
卷烟烟气/MAPK/信号通路/炎症/人支气管上皮细胞Key words
Cigarette smoke/MAPK/Pathway/Inflammation/Human bronchial epithelial cell分类
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XU Liangtao,LI Xiang,WANG Huiting,LIU Kejian,XIE Fuwei,LIU Huimin,XIE Jianping..MAPK信号通路调节卷烟烟气诱导的炎症因子的释放[J].烟草科技,2019,52(6):35-41,7.
