烟草科技2019，Vol.52Issue(6)：42-50,9.
高效液相色谱-三重四极杆质谱法同时测定烟叶中10种多酚类化合物
Simultaneous determination of ten polyphenols in tobacco leaves with high performance liquid chromatography-triple quadrupole mass spectrometry
摘要
Abstract
In order to study polyphenols in tobacco, a method for simultaneous determination of ten endogenous polyphenols in tobacco was developed with high performance liquid chromatography-triple quadrupole mass spectrometry(HPLC-TQMS). The ten polyphenols included kaempferol, cyanidin, delphinidin chloride, rhamnazin, chlorogenic acid, delphinidin 3-O-β-glucopyranoside chloride, procyanidine B2, keracyanin chloride, rutin, and scopoletin. The characteristic fragment ion peaks of the ten polyphenols were obtained by dynamic multiple reaction monitoring (DMRM). Tobacco mosaic virus (TMV)-infected tobacco samples were also included in the study. The results showed that: 1) The linear correlation coefficients (r) of the ten polyphenols were from 0.999 0 to 0.999 6. The limits of detection(LODs)for all the analytes were 1-200 μg/L, and the intra-day and inter-day relative standard deviations (RSDs) were 0.64%-8.72% and 1.05%-7.93% , respectively. The recoveries were in the range of 79.66%-112.74% . 2)The contents of kaempferol, cyanidin, delphinidin chloride, delphinidin 3-O-β-glucopyranoside chloride and rutin in TMV-infected tobacco samples were significantly higher(p＜0.01)than those in the control, and the rhamnazin content was significantly lower (p＜0.05) and the chlorogenic acid content was higher in TMV-infected tobacco than in the control. 3) The contents of procyanidine B2, keracyanin chloride, and scopoletin were not significantly affected by TMV infection. This method could be used to simultaneously determine a variety of endogenous polyphenols with good sensitivity, precision and recovery.关键词
烟草/多酚/高效液相色谱-三重四极杆质谱/烟草花叶病毒(TMV)/同时测定Key words
Tobacco/Polyphenol/High performance liquid chromatography-triple quadrupole mass spectrometry (HPLC-TQMS)/Tobacco mosaic virus(TMV)/Simultaneous determination分类
轻工纺织引用本文复制引用
LIU Pingping,CHEN Qiansi,LU Zishu,LUO Zhaopeng,LI Zefeng,ZHAI Niu,ZHENG Qingxia,LU Peng,CAO Peijian,ZHOU Huina..高效液相色谱-三重四极杆质谱法同时测定烟叶中10种多酚类化合物[J].烟草科技,2019,52(6):42-50,9.基金项目
国家烟草专卖局资助项目"典型香型烟草品质形成的代谢特征研究"[110201401012(JY-12)]、"烟草基因功能元件全景图构建及关键基因挖掘与调控节点研究"[110201601033(JY-07)]、"烟草叶片磷酸化蛋白质组检测方法建立"(902014CA0410)、"烟草干旱相关miRNA(NovelmiR233、NovelmiR294)的功能研究"(902017CA0200)、"烟草荧光细胞分选技术建立及在多酚代谢研究中的应用"(902018CA0250)、"烟草功能基因的细胞器及亚细胞结构定位体系的建立"(902014CA0440). （JY-12）
