医学新知杂志2019，Vol.29Issue(2)：150-154,5.DOI:10.3969/j.issn.1004—5511.2019.02.011
呼吸道合胞病毒非结构蛋白1感染对A549细胞let—7c—5p及miR—122—5p表达的影响
Expression of let-7c-5p and miR-122-5p in Nonstructural Protein I of Respiratory Syncytial Virus Infected A549 Cells
摘要
Abstract
Objective To study the alteration of let-7c-5p and miR-122-5p expression in A549 cells after infected with nonstructural protein I(NS1)of respiratory syncytial virus(RSV). Methods We cultured A549 cells to establish the infection model of NS1,and the control of empty vector group was also set up. Cells after 24 h and 72 h infection were collected and real-time PCR was performed to evaluate the alteration of let-7c-5p and miR-122-5p expression. Target gene prediction and function analysis were performed using bioinformatics software. Results Let-7c-5p expression elevated while miR-122 -5p expression decreased in NS1 infection group. Bioinformatics software predicted that target genes focused on apoptosis and immune response. Conclusion NS1 probably regulate cell apoptosis and suppress immune response by upregulating the expression of let-7c-5p and downregulating the expression of miR-122-5p.关键词
呼吸道合胞病毒/非结构蛋白/miRNAsKey words
Respiratory syncytial virus/Nonstructural protein/MiRNAs分类
医药卫生引用本文复制引用
Zeng Dongxin,Feng Shuwen,Wang Shouyi..呼吸道合胞病毒非结构蛋白1感染对A549细胞let—7c—5p及miR—122—5p表达的影响[J].医学新知杂志,2019,29(2):150-154,5.基金项目
国家自然科学基金资助(81670007) （81670007）
