中风与神经疾病杂志2019，Vol.36Issue(3)：200-203,4.
人参皂甙Rb1通过调控缝隙连接蛋白40治疗脑缺血再灌注损伤的机制
Regulation of Cx40 By GS-Rb1 in The Treatment For Cerebral ischemia-Reperfusion injury
摘要
Abstract
Objective To investigate the mechanism of protective effects of GS-Rb1 against Cerebral ischemia-repu-fusion injury in super acute phase with the expression of protein Cx40. Methods Cerebral ischemia- repufusion injury model were established by Sprague-Dawley rats at 3 months age,weight at 300~500 g,with clipping bilateral CCA and in-traperitoneal injection of GS-Rb1 or GS-Rb1+ PKA inhibitor H-89. One hunderd rats Rats were randomly divided into 5 groups,100 rats in total,20rats in each group:sham operation,treatment group (I/R8 hour+GS-Rb1),inhibition group(I/R8 hour+ GS-Rb1+ H-89),vehicle group ( I/R8 hour+ DMSO) and injury group (I/R 8 hour). Ten rats were selected for testing behavioral disorder by NSS and cerebral edema by measuring brain water content. Another ten rats in each group were sacrificed and glial tissue in hippocampus were obtained for detecting the level of Cx40 by western Western blot,NAP-DH oxidase activity by EFLA,IL-1 β,IL-10 and TNF-α by ELISA. Result NSS,brain water content,NAPDH oxidase ac-tivity,IL-1 β,IL-10,TNF-α and Cx40 in GS-Rb1 treatment group were lower than in vehicle group and injury group,PKA inhibitor H-89 was proved that it can could inhibit above protective effect of GS-Rb1. Conclusion Protective effect of GS-Rb1 to cerebral I/R injury is involved in PKA pathway to reduce the expression of Cx40,by which can reduce the oxidative stress,inflammatory factors and alleviate the injuries of form and function of brain after cerebral ischemia-reperfusion injury.关键词
缝隙连接40/人参皂甙Rb1/细胞激酶A/脑水肿/脑缺血再灌注损伤Key words
Cx40/GS-Rb1/PKA/Cerebraledema/Ischemirepufusioninjury分类
医药卫生引用本文复制引用
SHI Hong,REN Dabin,ZHENG Ping..人参皂甙Rb1通过调控缝隙连接蛋白40治疗脑缺血再灌注损伤的机制[J].中风与神经疾病杂志,2019,36(3):200-203,4.基金项目
[浦东新区科委民生项目(编号:PKJ2016-Y31) （编号:PKJ2016-Y31）
上海健康医学院种子基金项目(编号:SFP-18-21-13-007) （编号:SFP-18-21-13-007）
上海健康医学院种子基金项目(编号:SFP-18-21-13-005) （编号:SFP-18-21-13-005）
国家自然科学基金(编号:81701231) （编号:81701231）
上海市自然基金(编号:16ZR1431500)] （编号:16ZR1431500）
