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RNA干扰染色体空间重组因子1对宫颈腺癌放化疗敏感性的影响

XIE Zhaoguang XU Jinquan WEI Ling WANG Xingwu SUN Li WANG Xiangyu RAO Linli GUO Qiufen WU Qian CUI Chunhui ZHANG Tingting CHEN Jinlong JIA Jue

肿瘤药学2019，Vol.9Issue(1)：20-25,6.
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肿瘤药学2019，Vol.9Issue(1)：20-25,6.DOI:10.3969/j.issn.2095-1264.2019.01.05

RNA干扰染色体空间重组因子1对宫颈腺癌放化疗敏感性的影响

Effects of siRNA Targeting of Rsf-1/HBXAP Gene on the Radiosensitivity and Chemosensitivity of Cervical Adenocarcinoma★

XIE Zhaoguang 1XU Jinquan 2WEI Ling 3WANG Xingwu 2SUN Li 2WANG Xiangyu 2RAO Linli 1GUO Qiufen 4WU Qian 5CUI Chunhui 2ZHANG Tingting 2CHEN Jinlong 2JIA Jue2

作者信息

  • 1. School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences, Jinan, Shandong,250022, China
  • 2. Department of Gynecologic Oncology, Shandong Provincial Cancer Hospital, Shandong University, Jinan, Shandong, 250117, China
  • 3. Institute of Biomedical Sciences, National Sun Yatsen University, Kaohsiung, Taiwan, Taiwan, 80424, China
  • 4. Department of Obstetrics and Gynecology, Fengxian District Central Hospital, Shanghai, 201400, China
  • 5. Department of Obstetrics and Gynecology, Huaiyin District People’s Hospital, Jinan, Shandong Province, Jinan, Shandong, 250000, China
  • 折叠

摘要

Abstract

Objective To investigate the effects of siRNA interference with chromosomal space recombination factor 1 (Rsf-1/HBXAP) on the chemosensitivity of cervical adenocarcinoma cells, and provide experimental evidence for gene-targeted therapy of cervical adeno-carcinoma. Methods Human cervical adenocarcinoma HeLa cells were transfected with Rsf-1/HBXAP-specific small interfering RNA (siRNA). The Rsf-1/HBXAP mRNA expression in HeLa cells was detected by real-time quantitative polymerase chain reaction (qRT-PCR). The proliferation of HeLa cells was detected by CCK-8 assay. The clone formation of HeLa cells was detected by plate cloning assay. The apoptosis of HeLa cells was detected by flow cytometry. Results Rsf-1/HBXAP-specific siRNA-1 significantly inhibited the expression of the Rsf-1/HBXAP gene. The IC50 of paclitaxel against siRNA-1 in the Rsf-1/HBXAP and non-interfering groups was 7.24 nM [95% CI:(3.14, 18.60)] and 3.66 nM [95% CI: (1.65, 7.86)], respectively. Plate cloning experiments showed that siRNA-1 interference with Rsf-1/HBXAP gene improved the radiosensitivity. Flow cytometry results showed that siRNA-1 interference with Rsf-1/HBXAP gene accelerated the apoptosis of HeLa cells. Conclusion The down-regulation of Rsf-1/HBXAP gene expression may increase the sensitivity of cervical ad-enocarcinoma cells to chemoradiotherapy. It may become a potential target for the treatment of cervical adenocarcinoma.

关键词

宫颈腺癌/染色体空间重组因子1/小分子干扰RNA/放疗敏感性/化疗敏感性

Key words

Cervical adenocarcinoma/ Chromosome spatial recombination factor 1/ Small interference RNA/ Radiosensitivity/ Chemo-sensitivity

分类

医药卫生

引用本文复制引用

XIE Zhaoguang,XU Jinquan,WEI Ling,WANG Xingwu,SUN Li,WANG Xiangyu,RAO Linli,GUO Qiufen,WU Qian,CUI Chunhui,ZHANG Tingting,CHEN Jinlong,JIA Jue..RNA干扰染色体空间重组因子1对宫颈腺癌放化疗敏感性的影响[J].肿瘤药学,2019,9(1):20-25,6.

基金项目

基金项目:山东省重点研发计划(2016WS0562) （2016WS0562）

山东省医药卫生科技发展计划(2016WSA18028) （2016WSA18028）

山东省卫生厅课题(2011HZ097) （2011HZ097）

山东省自然基金课题(ZR2012HM010). （ZR2012HM010）

肿瘤药学

OACSTPCD

2095-1264

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