肿瘤药学2019，Vol.9Issue(1)：35-39,44,6.DOI:10.3969/j.issn.2095-1264.2019.01.07
COX2抑制剂联合KDM1A基因对肺癌A549细胞凋亡的诱导作用研究
Effects of COX2 Inhibitor Combined with KDM1A Gene on the Apoptosis of A549 Lung Cancer Cells in Vitro
WU Jun 1WANG Xiuli 1ZUO Xuejiao 1LEI Xingyun 1LIU Wen1
作者信息
- 1. The Second Affiliated Hospital of Army Medical University, Chongqing, 400037, China
- 折叠
摘要
Abstract
Objective To investigate the effect of COX2 inhibitor celecoxib or KDM1A siRNA on the proliferation and apoptosis of A549 lung cancer cells. Methods LipofectamineTM2000 was used as a carrier. KDM1A siRNA was transfected into human lung adenocarcinoma A549 cells, and the KDM1A expression in transfected A549 cells was detected by Western blot. After treated with celecoxib or si-KDM1A, the A549 cells were divided into NC group, celecoxib group, si-KDM1A group, celecoxib + si-KDM1A group. The cell viability was de-tected by CCK8 assay, and the apoptosis rate was detected by flow cytometry. The content of ROS was detected through the H2DCFHDA fluorescent probe. The expression of STAT3, p-STAT3, PCNA and Bax proteins were detected by Western blot. Results The expression of KDM1A in A549 cells after transfected with si-KDM1A was significantly inhibited, and there was significant difference when compared with the NC group (P<0.05). Celecoxib or si-KDM1A both could significantly inhibit A549 cell viability, induce apoptosis, increase the content of ROS in cells, down-regulate the expression of p-STAT3 and PCNA and up-regulate the expression of Bax. All differences were statisti-cally significant when compared with NC group (P<0.05). The combination of celecoxib and si-KDM1A had much greater effects on cell proliferation, apoptosis and STAT3 signaling pathway. The results of cell viability, apoptosis rate, ROS content and expression of p-STAT3, PCNA and Bax were significantly different from those in celecoxib group and si-KDM1A group (P<0.05). Conclusion COX2 inhibitors or KDM1A siRNA both inhibit A549 cell viability and induce cell apoptosis. Their combination was stronger than single use. The mechanism may be related to the increase of intracellular ROS content and the inhibition of STAT3 signaling pathway.关键词
COX2抑制剂/KDM1A基因/肺癌/凋亡/STAT3信号通路Key words
COX2 inhibitors/ KDM1A gene/ Lung cancer/ Apoptosis/ STAT3 signaling pathway分类
医药卫生引用本文复制引用
WU Jun,WANG Xiuli,ZUO Xuejiao,LEI Xingyun,LIU Wen..COX2抑制剂联合KDM1A基因对肺癌A549细胞凋亡的诱导作用研究[J].肿瘤药学,2019,9(1):35-39,44,6.
