肿瘤药学2019，Vol.9Issue(1)：40-44,5.DOI:10.3969/j.issn.2095-1264.2019.01.08
羟喜树碱对外源野生型p53转染宫颈癌细胞作用效果的研究
Effect of Hydroxycamptothecin on the Transfection of Wild-type p53 into Cervical Cancer Cells★
摘要
Abstract
Objective To investigate the effects of Hydroxycamptothecin (HCPT) on the cervical cancer cells transfected with wild-type p53. Methods HeLa cells were cultured in vitro, and some were transfected with the plasmid pcDNA3.1-HA-EGFP (negative control group) or the target plasmid pcDNA3.1-HA-TP53 (experimental group). Then the cells were divided into 5 groups: blank control group, sol-vent group, HCPT group, p53 group and HCPT+p53 group. The expression of the p53 gene was detected by qRT-PCR, CCK-8 assay was used to detect the viability of cells treated with different concentrations of HCPT. The apoptosis rates of different groups were measured by flow cytometry. Results Under the fluorescent microscope, there was obvious green fluorescence in the cells, indicating that the plasmid was successfully transfected. After the HeLa cells were transfected with the target plasmid, the mRNA expression of p53 gene was up-regulated along with the increase of the transfection plasmid dose. Compared with the blank and negative control groups, the viability of cells in exper-imental groups was inhibited in a HCPT-dose dependent manner, and there were statistical differences when it treated with different doses of HCPT (P<0.05). Compared with the blank control group and solvent group, the apoptosis rates of HCPT group and p53 group were increased (P<0.05), and that of the p53+HCPT group was significantly higher than that of the other experimental groups (P<0.05). Conclusion The introduction of exogenous wild-type p53 gene into cervical cancer HeLa cells can significantly enhance the effect of HCPT in inhibiting the proliferation of cervical cancer cells and promoting its apoptosis.关键词
羟喜树碱/p53/宫颈癌/凋亡Key words
Hydroxycamptothecin/ p53/ Cervical cancer/ Apoptosis分类
医药卫生引用本文复制引用
QI Qingxia,PAN Ying,HE Quanzhong,SUN Li,LIU Pingping..羟喜树碱对外源野生型p53转染宫颈癌细胞作用效果的研究[J].肿瘤药学,2019,9(1):40-44,5.基金项目
基金项目:新乡市重点科技攻关项目(ZG15016). （ZG15016）
