肿瘤药学2019，Vol.9Issue(2)：219-225,237,8.DOI:10.3969/j.issn.2095-1264.2019.02.09
miR-148a-3p靶向调控PTEN基因表达对黑色素瘤细胞生物学特性的影响
MiR-148a-3p Down Regulation Inhibits Cell Proliferation, Migration and Invasion and Promotes Cell Apoptosis by Enhancing PTEN in Melanoma
摘要
Abstract
Objective To investigate miR-148a-3p in target regulating the expression of PTEN and its effects on cell proliferation, migration and apoptosis in melanoma. Methods The melanoma tissues and adjacent normal tissues 5 cm away from the tumor edge were collected from 90 cases of melanoma patients. Expression of miR-148a-3p and PTEN in both melanoma tissue and adjacent normal tissue were determined. Next, after siRNA of miR-148a-3p or PTEN was transfected into melanoma cell line SK-MEL-3, the cell proliferation, invasion, migration and apoptosis of SK-MEL-3 were detected using CCK-8 assay, Transwell assay and flow cytometry respectively. Re-sults Initially, it was observed that miR-148a-3p was up-regulated while PTEN was down-regulated in melanoma tissues, when compared with adjacent normal tissues (P<0.05). Besides, expression of miR-148a-3p was related with melanoma lymph node metastasis and tumor node metastasis (TNM) stage (P<0.05). The PTEN expression was not only related with melanoma lymph node metastasis and TNM stage, but also tumor differentiation (P<0.05). In addition, miR-148a-3p siRNA elevated PTEN expression, and thus suppressed cell prolifera-tion, invasion and migration and enhanced cell apoptosis in melanoma (P<0.05). After transfection of miR-148a-3p siRNA, PTEN siRNA promoted cell proliferation, invasion and migration and suppressed cell apoptosis in melanoma. Conclusion In conclusion, miR-148a-3p expression was negatively correlated with the PTEN expression. It may promote proliferation, invasion and migration, and inhibit apoptosis of SK-MEL-3 cells by down-regulating PTEN in melanoma.关键词
黑色素瘤/miR-148a-3p/PTEN/增殖/凋亡Key words
Melanoma/MicroRNA-148a-3p/PTEN /Proliferation/Invasion and migration/Apoptosis分类
医药卫生引用本文复制引用
HUANG Huiyun,SHEN Erdong,TANG Siqing,SU Yuqi..miR-148a-3p靶向调控PTEN基因表达对黑色素瘤细胞生物学特性的影响[J].肿瘤药学,2019,9(2):219-225,237,8.基金项目
湖南省自然科学基金面上项目(2017JJ2260). （2017JJ2260）
