摘要
Abstract
Objective To investigate the effect of allicin on autophagy of endoplasmic reticulum (ER) in apoptosis of gastric cancer SGC-7901 cells. Methods Gastric cancer SGC-7901 cells were divided into control group (normal culture), low, medium and high doses of allicin group (SGC-7901 cells were added with 12, 24, 48 μg·mL-1 allicin for culture). The cell proliferation rate of each group was detected by CCk-8 method at 24h, 48h and 72h after culture. TUNEL was used to detect apoptosis, and Immunofluorescence assay was used to detect the colocalization of LC3 and Sec61 proteins in each group. Western blot was used to detect the expression of GRP78, PERK, p-PERK, eIF2a, ATF-4, CHOP, Beclin-1, LC3I and LC3II proteins. Results Compared with the control group, the co-localizations of ATG8 and Sec61 protein in the allicin groups were de-creased along with the increase of the dose of allicin, while the proliferation inhibition rate and the apoptotic rate were increased along with the in-crease of the dose (P<0.01). Moreover, the expression of GRP78, PERK, p-PERK, eIF2a, ATF-4 and CHOP in low, medium and high dose groups were increased in a dose-dependent manner(P<0.01). The expression of Beclin-1 was decreased with the increasing dose, while the ratio of LC3I and LC3II rose along with the increasing dose (P<0.01). Conclusion Allicin could inhibit the proliferation of gastric cancer SGC-7901 cells by in-hibiting autophagy in the endoplasmic reticulum of gastric cancer SGC-7901 cells and inducing endoplasmic reticulum stress.关键词
大蒜素/胃癌/增殖/内质网应激/内质网自噬Key words
Allicin/Gastric cancer/Proliferation/Endoplasmic reticulum stress/Endoplasmic reticulum autophagy分类
医药卫生
