烟草科技2019，Vol.52Issue(8)：9-15,7.
外源葡萄糖对高温强光下旺长期烤烟光合特性及碳氮代谢关键酶活性的影响
Effects of exogenous glucose on photosynthesis and key enzyme activities in carbon and nitrogen metabolism of flue-cured tobacco at fast-growing stage under high temperature and high light intensity
摘要
Abstract
In order to investigate the regulation mechanism of exogenous glucose on the photosynthesis mechanism and carbon and nitrogen metabolism of flue-cured tobacco leaves at fast-growing stage under high temperature and high light intensity, a pot experiment in an artificial climate chamber was conducted by simulating the high temperature and high light intensity environment of flue-cured tobacco at fast-growing stage in central Henan tobacco-planting areas. The effects of exogenous glucose (0.5 mmol/L) on the contents of photosynthetic pigments, including chlorophyll a (Chla), chlorophyll b (Chlb), total chlorophyll [Chl(a + b)], carotenoids (Car), the photosynthetic parameters, including net photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (E), intercellular CO2 concentration (Ci), chlorophyll fluorescence parameters, including initial fluorescence (Fo), nonphotochemical quenching coefficient (NPQ), actual photochemical quantum efficiency of PSⅡ ( Φ PSⅡ), photosynthetic electron transfer efficiency (ETR), photochemical quenching coefficient (qp), maximum photochemical efficiency of PSⅡ (Fv/Fm), glutamine synthase activity (GS) and 1,5-diphosphate ribulose carboxylase (Rubisco) activity of flue-cured tobacco were studied. The results showed that spraying exogenous glucose under high temperature and high light intensity significantly promoted the contents of Chla, Chlb, Chl(a+b) and Car, noticeably raised Pn, Gs, E, remarkably reduced Ci, Fo and NPQ; Φ PSⅡ, ETR, qp were increased significantly, in which the increase extent of Fv/Fm was relatively smaller. Furthermore, the activities of GS and Rubisco were improved. Therefore, spraying exogenous glucose under high temperature and high light intensity promotes the photosynthetic performance, carbon and nitrogen metabolism balance and the growth of tobacco, and alleviates the adverse effects of high temperature and high light intensity stress on tobacco.关键词
烤烟/外源葡萄糖/光合特性/高温/强光/GS活性/Rubisco活性Key words
Flue-cured tobacco/Exogenous glucose/Photosynthetic characteristic/High temperature/High light intensity/GS activity/Rubisco activity分类
农业科技引用本文复制引用
张艾改,刘国顺,云菲,张林..外源葡萄糖对高温强光下旺长期烤烟光合特性及碳氮代谢关键酶活性的影响[J].烟草科技,2019,52(8):9-15,7.基金项目
河南省高等学校重点科研项目计划"低光胁迫下喜光植物光合碳同化与土壤碳积累的适应性调节机制"(15A210037) （15A210037）
河南省科技发展计划(高新技术领域)项目"改性生物炭对植烟土壤重金属污染及养分流失的阻控效应及调控机理"(172102210306). （高新技术领域）
