医学新知杂志2019，Vol.29Issue(4)：411-413,3.DOI:10.3969/j.issn.1004﹣5511.2019.04.019
HPLC法用于溃疡灵胶囊中人参皂苷Rg1 含量的测定
Determination of Ginsenoside Rg1 in Kuiyangling Capsule by HPLC
潘彦荣 1刘艳霞 1常晓平1
作者信息
- 1. 461000许昌,许昌市食品药品检验检测中心
- 折叠
摘要
Abstract
Objective To detect the content of ginsenoside Rg1 in Kuiyangling capsule to control the quality of the product and enhance the binding force of the standard.Methods The content of ginsenoside Rg1 in Kuiyangling capsule was determined by HPLC.Chromatographic conditions were as follows:chromatographic column Hypersil BDS C18(250 mm×4.6 mm,5μm);mobile phase acetonitrile-0.05%phosphoric acid solution(21 :79),detection wave﹣length:203 nm,column temperature 30℃,flow rate 1.0 ml／min.The theoretical plate number calculated by ginsen﹣oside Rg1 could not be less than 5000.Results Ginsenoside Rg1 had a good linear relationship within the rage 0.852～4.260 μg(r＝0.9999).The average recovery rate of ginsenoside Rg1 was 97.86%,RSD＝1.65%.Conclusion HPLC method is stable,reliable,accurate and reproducible,which can be used for the quality control of Kuiyangling capsule.关键词
溃疡灵胶囊/人参皂苷Rg1 /HPLC法Key words
Kuiyangling capsule/Ginsenoside Rg1 /HPLC method分类
中医学引用本文复制引用
潘彦荣,刘艳霞,常晓平..HPLC法用于溃疡灵胶囊中人参皂苷Rg1 含量的测定[J].医学新知杂志,2019,29(4):411-413,3.
