肿瘤预防与治疗2019，Vol.32Issue(9)：773-780,8.DOI:10.3969/j.issn.1674-0904.2019.09.005
肿瘤微环境细胞亚群基因标签预测复发转移性头颈鳞状细胞癌EGFR单抗疗效*
Gene Signatures of Cell Subpopulations in Tumor Microenviroment Pre-dict Response to EGFR Monoclonal Antibodies in Recurrent and Metastat-ic Squamous Cell Carcinoma of Head and Neck
摘要
Abstract
Objective: Objective: To develop a model for predicting response to anti-epidermal growth factor receptor ( anti-EGFR) antibody by using feature genes of cell subpopulations in the tumor microenvironment of recurrent and metastat-ic squamous cell carcinoma of the head and neck. Methods: Gene Expression Omnibus datasets with records of progression free survival after treated with anti-EGFR antibody and gene expression profiles evaluated by microarray assay were retrieved. The feature genes peculiar to each of cell subpopu-lations in the tumor microenvironment was obtained from previ- ously published articles. Differentially expressed genes were firstly identified between the long progression free survival (PFS) group and the short PFS group in the discovery set and used to calculate sensitive indexes of each cell subpopulation. Least absolute shrinkage and selection operator was used to establish the anti-EGFR antibody sensitivity score (EASS) which was further verified in the validation set. Results: The median EASS deduced from the nine cell subpopulations was 30. 73 (26. 24-41. 29) in the discovery set GSE65021. The long PFS group was significantly discriminated from the short PFS group through EASS revealed by ROC analysis (AUC: 1. 000, 95% CI: 1. 000-1. 000, P<0. 001). In the validation set GSE102995, the EASS remained an independent prognostic factor for PFS after adjusted for stage (IV vs II-III) and per-formance status score (HR=0. 647,95% CI:0. 503-0. 831,P=0. 001). The consensus index of EASS for predicting PFS was 0. 755 (95% CI: 0. 719-0. 791), which was significantly higher than that of stage 0. 542 (95% CI: 0. 336-0. 748) (P=0. 032). Conclusion: The EASS is efficient to predict the response to anti-EGFR antibody in the recurrence and metas-tasis of squamous cell carcinoma of the head and neck, which is worthy of further study and validation.关键词
头颈部鳞癌/EGFR单抗/肿瘤微环境/细胞亚群Key words
Head and neck squamous cell carcinoma/EGFR monoclonal antibody/Tumor microenvironment/Cell sub-population分类
医药卫生引用本文复制引用
黄环,张琴,肖何,王阁..肿瘤微环境细胞亚群基因标签预测复发转移性头颈鳞状细胞癌EGFR单抗疗效*[J].肿瘤预防与治疗,2019,32(9):773-780,8.基金项目
国家自然科学基金面上项目(编号:81572959) This study was supported by The General Program of National Natural Science Foundation of China ( NO. 81572959) . （编号:81572959）
