医学分子生物学杂志2019，Vol.16Issue(5)：474-477,4.DOI:10.3870/j.issn.1672-8009.2019.05.011
抑制TC-1表达对胶质瘤细胞增殖、凋亡、迁移和侵袭的影响
Effects of Inhibiting TC-1 Expression on Proliferation, Apoptosis, Migration and Invasion of Glioma Cells
冯毅 1黄琦丹 1陈家康1
作者信息
- 1. 柳州市工人医院神经外科 广西壮族自治区柳州市, 545000
- 折叠
摘要
Abstract
Objective To investigate the effects of inhibiting TC-1 expression on proliferation, apoptosis, migration and invasion of glioma cells and the underlying mechanisms. Methods Ex-pressions of TC-1 mRNA in glioma U251 cells and normal brain tissue cells were detected by qRT-PCR. The si-NC group ( transfected si-NC ) and the si-TC-1 group ( transfected si-TC-1 ) were transfected into U251 cells by liposome method. Protein expression was detected by Western blotting;cell proliferation by using cell counting kit-8 ( CCK-8) ; apoptosis by flow cytometry; cell migra-tion and invasion by Transwell assay. Results Compared with normal brain tissue cells, the expres-sions of TC-1 mRNA was significantly increased in glioma U251 cells ( P< 0. 05) . In comparison with the si-NC group, the expression of TC-1 mRNA and protein was significantly decreased in the si-TC-1 group, the expression of Bax protein was significantly increased, and the expression of Bcl-2 protein was significantly decreased; cell viability, cell migration and invasion number were signif-icantly decreased, and apoptosis rate was significantly increased ( P < 0. 05) . Conclusion In-hibiting TC-1 expression can inhibit the proliferation, migration, invasion and promote apoptosis of glioma U251 cells. The mechanism may be related to the expression of Bax and Bcl-2 proteins and they might serve as new targets for the prevention and treatment of glioma.关键词
TC-1/胶质瘤/增殖/凋亡/迁移/侵袭Key words
TC-1/glioma/proliferation/apoptosis/migration/invasion分类
医药卫生引用本文复制引用
冯毅,黄琦丹,陈家康..抑制TC-1表达对胶质瘤细胞增殖、凋亡、迁移和侵袭的影响[J].医学分子生物学杂志,2019,16(5):474-477,4.
