广西医科大学学报2019，Vol.36Issue(9)：1391-1396,6.DOI:10.16190/j.cnki.45-1211/r.2019.09.001
YKL-40基因沉默对裸鼠子宫内膜癌移植瘤生长及CD68表达的影响
Effect of YKL-40 gene silencing on the tumor growth of human endometrial carcinoma transplanted in nude mice and tumor CD68 expression
摘要
Abstract
Objective:To investigate the effects of YKL-40 gene silencing on the growth of the transplanted tumor and expression of CD68 in tissue of the endometrial carcinoma of nude mice.Methods:We silenced the YKL-40 gene in human endometrial carcinoma (HEC)-1A cells using the small interfering (si)RNA technique and divided the HEC 1A cells into blank control group,mock-treatment group and YKL-40-siRNA group.The HEC-1A cells were injected subcutaneously into the armpits of 4 week-old BALB/c nude mice to establish a nude mouse endometrial cancer model.The growth of transplanted tumors in nude mice was observed.The expressions of YKL-40 and CD68 in tumor tissues were detected by immunohistochemistry.Results:The tumor volume of YKL-40-siRNA transfection group was lower than that of nonsense siRNA transfection group and blank control group.The expression level of YKL-40 was significantly decreased in the tumor tissues of the nude mice in the group subjected to the injection of recombinant lentiviral vectors YKL-40-siRNA (P ＜0.05).Furthermore,the inhibition of YKL-40 expressiondown-regulated the expression of CD68 in the tumor tissues (P＜0.05).Spearman correlation analysis showed that the expression of YKL-40 was positively correlated with the CD68 expression in the tumor tissue of the nude mice.Conclusion:Inhibition of YKL-40 expressionc ould prevent transplanted endometrial carcinoma growth and reduce the expression of CD68.关键词
子宫内膜癌/裸鼠/YKL-40/CD68/免疫组化Key words
endometrial cancer/nude mice/YKL-40/CD68/immunohistochemistry分类
医药卫生引用本文复制引用
罗钦,范江涛,李莉莉,陈红燕..YKL-40基因沉默对裸鼠子宫内膜癌移植瘤生长及CD68表达的影响[J].广西医科大学学报,2019,36(9):1391-1396,6.基金项目
The present study was supported by the National Natural Science Foundation of China(No.81360388),Guangxi Natural Science Foundation (No.2016 GXNSFAA380258),Self-financing Project of Guangxi Health and Family Planning Commission (No.z2016282) and Guangxi Graduate Education Innovation Program (No.YC-SW2017104). （No.81360388）
