临床神经外科杂志2019，Vol.16Issue(5)：390-396,7.DOI:10.3969／j.issn.1672-7770.2019.05.005
高良姜黄素促进内皮细胞自噬及抑制损伤内膜过度增生的作用机制
Mechanism of curcumin in promoting endothelial cells autophagy and inhibiting vascular intimal hyperplasia
摘要
Abstract
Objective To explore the mechanism of curcumin (Cur) in enhancing the level of autophagy inside endothelial cells (ECs) and inhibiting damaged vascular intimal hyperplasia ( IH). Methods In vitro, Human Umbilical Vein Endothelial Cell ( HUVECs ) was divided into four groups: Control, 3-MA, Cur and Rapa group.Acridine orange(AO) staining and Lyso-Tracker Red method were used to observe the acidic compartment accumulation. Western-blot and immunofluorescence were used to evaluate the protein levels of microtubule-associated protein-Ⅱ(LC3-Ⅱ), p62, Akt and mTOR.In vivo, rat carotid artery endarterium injury model was created by balloon compression , and 60 SD rats were divided into Sham , injured+vehicle, 3-MA, Cur and Rapa group.The protein levels of LC3-Ⅱ, p62, Akt and mTOR in carotid artery tissue were detected by Western blot.HE staining was used to measure the IH thickness and effective lumen area of each group.Results In vitro, Cur and Rapa treatments significantly increased the acidic compartment accumulation , the protein level of LC3-Ⅱand inhibited the protein expression of p62, Akt and mTOR in HUVECs compared with control group.In vivo, protein level of LC3-Ⅱ markly increased and p62, Akt and mTOR decreased slightly after endometrial injury and reversed by Cur and Rapa treatments.In addition, HE staining presented obvious IH and vascular stenosis was observed after injury in 3 weeks, and Cur and Rapa both markedly suppressed IH and alleviated vascular stenosis.Finally, 3-MA treatment showed an opposite effect both in vitro and in vivo. Conclusion Cur increases the level of ECs autophagy and inhibits IH after vascular injury , possibly by inhibiting the AKt／mTOR signaling pathway in ECs.关键词
高良姜黄素/人脐血管内皮细胞/自噬/内膜增生/ Akt／mTOR信号通路Key words
curcumin/ human umbilical vein endothelial cells / autophagy/ intimal hyperplasia/ Akt／mTOR signaling pathway分类
医药卫生引用本文复制引用
汪洋,董永飞,鲍得俊,程传东,齐印宝,魏祥品,牛朝诗..高良姜黄素促进内皮细胞自噬及抑制损伤内膜过度增生的作用机制[J].临床神经外科杂志,2019,16(5):390-396,7.基金项目
安徽省自然科学基金青年项目(1708085QH174) （1708085QH174）
