临床神经外科杂志2019，Vol.16Issue(5)：420-424,5.DOI:10.3969／j.issn.1672-7770.2019.05.011
抑制 ROCKⅡ下调丝切蛋白1表达提高胶质瘤细胞的放射敏感性
Improve U251 glioma cell radiosensitivity and down-regulate cofilin1 via inhibiting ROCKⅡ
摘要
Abstract
Objective To clarify the potential mechanism of CFL1-mediated radioresistance and the association of CFL1 upstream regulators with cell motility after irradiation in human glioma cells.Methods After inhibiting ROCKⅡ, the alteration of radiosensitivity and CFL 1 expression level in both normal U251 and radioresistant U251 ( RR-U251) cells were respectively evaluated. The cell radiosensitivity was assessed through cell vitality , invasion and migration experiments after irradiation.Results The results showed that the cell vitality , migration and invasion capabilities of RR-U251 cells significantly decreased with radiosensitivity significantly improved via inhibiting ROCKⅡ.Down-regulation of ROCKⅡ could simultaneously decrease CFL 1 level in glioma cells. Conclusion The present study indicates the involvement of ROCK Ⅱ in CFL1-mediated radioresistant phenotype and provides potential biomarkers for improvement of further clinical radiotherapy.关键词
胶质瘤/放射敏感性/丝切蛋白1/ ROCKⅡ/ U251细胞Key words
glioma/ radiosensitivity/ cofilin1/ ROCKⅡ/ U251 cells分类
医药卫生引用本文复制引用
仲钰婕,肖红,尤为,赵梦洁,肖勇,王臻,王冉冉,钱春发,胡新华,刘宏毅..抑制 ROCKⅡ下调丝切蛋白1表达提高胶质瘤细胞的放射敏感性[J].临床神经外科杂志,2019,16(5):420-424,5.基金项目
国家自然科学基金( NSFC81172390) （ NSFC81172390）
南京医学科技发展基金(ZDX16011) （ZDX16011）
