临床神经外科杂志2019，Vol.16Issue(5)：425-429,5.DOI:10.3969／j.issn.1672-7770.2019.05.012
NR2 C2 基因在泌乳素瘤的表达及其意义
Expression and significance of NR2C2 gene in prolactinoma
摘要
Abstract
Objective To testify the expression of nuclear receptor subfamily 2 group C member 2 ( NR2C2) in the prolactinomas, and analyze the relationship between NR 2C2 and clinical features.Methods Immunohistochemical staining and Western blotting were used to detect the expression of NR2C2 in 46 prolactinoma patients.The relationship between NR2C2 and clinical features was analyzed by follow-up data.RNA interference technique ( RNAi) knocked down the NR2C2 level in MMQ cells, and cell viability was detected by cell proliferation assay.Flow cytometry was used to detect the apoptosis level by Annexin V／PI staining.Results The patients were divided into invasive group and non-invasive group according to Knosp classification.The H-score of NR2C2 in the invasive group was 148.2 ±42.7, and 45.2 ±28.72 in the non-invasive group( P＜0.01).The level of NR2C2 was positively correlated with tumor size ( r＝0.327,P＝0.039), and there was higher NR2C2 level in patients with loose granules than those in dense granules(P＝0.003).Western blot experiments filtered the fragments Sh-B (23.4%) and Sh-C (34%) compared to NR2C2 protein level in control MMQ cell (P＜0.01).The cell viability of Sh-B group reduced 24.8%, 39.2%and 48.3%, and Sh-C group was 19.1%, 26.4%and 32.5%compared to control cell after 24 h, 48 h and 72 h transfection( all P＜0.05).After 24 h RNAi, Annexin V positive cells were (10.3 ±3.1 )% in the Sh-B group and ( 8.5 ±2.3 )% in Sh-C group, and PI positive cells were (7.3 ±2.1)% and (5.6 ±1.8)% separately ( all P＜0.05). Conclusion NR2C2 is closely related to the tumorigenesis and development of prolactin adenoma.关键词
泌乳素瘤/核受体亚家族2c成员2/凋亡/细胞增殖Key words
prolactinomas/ nuclear receptor subfamily 2 group C member 2/ apoptosis/cell proliferation分类
医药卫生引用本文复制引用
杨晶晶,王大维,陈一元,刘潜,张亚卓,高华..NR2 C2 基因在泌乳素瘤的表达及其意义[J].临床神经外科杂志,2019,16(5):425-429,5.基金项目
国家自然科学基金( 81771489 ) （ 81771489 ）
北京市自然科学基金(7162035) （7162035）
北京市卫计委高层次人才计划(2015 3 040) （2015 3 040）
