动物营养学报2019，Vol.31Issue(8)：3561-3570,10.DOI:10.3969/j.issn.1006⁃267x.2019.08.017
饲粮粗蛋白质水平对断奶仔猪消化能力、结肠氨氮含量和炎症损伤的影响
Effects of Dietary Crude Protein Level on Digestive Ability, Colonic Ammonia Nitrogen Content and Inflammatory Injury of Weaned Piglets
摘要
Abstract
To investigate the effects of dietary crude protein ( CP) level on digestive ability, colonic ammonia nitrogen ( AN) content and inflammatory injury of colon of weaned piglets, ninety?six 25?day?old weaned pig?lets were selected and randomly divided into four groups after 7 days of adaptive feeding. The piglets were fed diets with 18%, 20%, 22% and 24% CP levels, respectively. Four piglets were selected in each group, and collected blood and slaughtered after feeding 6, 24, 48, 72 and 96 h. The samples were used for measuring pH, pepsin and duodenal chymotrypsin activities, colonic AN content, interleukin ( IL)?1β and IL?10 contents in serum and colon. The results showed as follows: 1) dietary CP levels had no significant effect on growth performance of weaned piglets (P＞0.05). 2) The pH in gastric contents in 24% CP group was significantly higher than that in 20% CP group ( P＜0.05 or P＜0.01) except for feeding 48 h; the activities of pepsin and duodenal chymotrypsin in 24% CP group were lower than those in 20% CP group during the whole experiment period: the difference in activity of pepsin was significant at 6 and 72 h ( P＜0.05) , and the difference in activ?ity of duodenal chymotrypsin was significant at 24 and 48 h ( P＜0.05).3) The content of AN in colon in 24%CP group was higher than that in 18% and 20% CP groups during the experiment except for feeding 6 h, and the difference was significant at 24 and 72 h ( P＜0.05 or P＜0.01). 4) The colonic IL?1β content in 24% CP group was significantly higher than that in 20% CP group at 26 to 96 h ( P＜0.05 or P＜0.01). During the whole experiment period, the colonic IL?10 content in 24% CP group was lower than that in 20% CP group, and the difference was significant at 24 and 72 h ( P＜0.05 or P＜0.01). 5) The correlation analysis showed that there was a significant negative correlation between chymotrypsin activity and AN content in colon ( P＜0.01) , significant positive and negative correlation between AN and IL?1β contents in colon, IL?1β and IL?10 contents in colon, respectively ( P＜0.05). In conclusion, feeding the diet with 20% CP level to weaned piglets can reduce the content of AN in colon by increasing the activity of chymotrypsin, and decrease the content of IL?1β, increase content of IL?10 in colon, thus reducing the inflammatory injury of colon of weaned piglets. [ Chinese Journal of Animal Nutrition, 2019, 31(8) :3561?3570]关键词
断奶仔猪/粗蛋白质水平/消化能力/氨氮/炎症损伤Key words
weaned piglets/crude protein level/digestive ability/ammonia nitrogen/inflammatory injury分类
农业科技引用本文复制引用
董彦强,邓俊良,樊皓月,姚淑华,王昱茜,姚任杰,孔祥雨,杨菊,陈代文,任志华..饲粮粗蛋白质水平对断奶仔猪消化能力、结肠氨氮含量和炎症损伤的影响[J].动物营养学报,2019,31(8):3561-3570,10.基金项目
国家"十三五"重点研发计划"猪碳水化合物、蛋白质和能量代谢失衡防控技术研究"项目( 2016YFD0501204) （ 2016YFD0501204）
四川农业大学双支计划(03570806) （03570806）
