动物营养学报2019，Vol.31Issue(8)：3651-3663,13.DOI:10.3969/j.issn.1006⁃267x.2019.08.026
不同氮利用率山羊瘤胃细菌结构与组成的差异
Differences of Bacterial Structure and Composition in Rumen of Goats with Different Nitrogen Utilization
摘要
Abstract
The objective of this study was to compare the differences in the bacterial structure and composition in rumen of goats with different nitrogen utilization. Thirty goats were chosen as the experimental animals, ac?cording to the data of metabolic test, goats were chosen as the high nitrogen utilization ( HNU) group and low nitrogen utilization ( LNU) group. Rumen contents of each goat in 2 groups were collected after slaughter and the differences of their bacterial structure and composition were analyzed and compared by high?throughput se?quencing technique, and 7 proteolytic bacteria were quantitatively analyzed and compared by real?time PCR. The results showed as follows: 1) there were obvious individual differences in the nitrogen utilization in 30 goats, the average nitrogen utilization of HNU group and LNU group was ( 56.68 ± 5.54 )% and ( 34.25 ± 4.21)%, and there was significant difference between groups ( P＜0.01). 2) The differences of rumen micro?organisms structure in HNU group and LNU group were different at the genus level. The relative abundances of Prevotella 1, Succiniclasticum, p?1088?a5_gut_group in HNU group were significantly lower than those in LNU group ( P＜0.05) , and the relative abundances of Lachnospiraceae XPB_1014 group, Ruminococcus 1, Ruminococcaceae UCG 004, Ruminobacter, Family_ⅩⅢ_UCG_ 002 and Anaeroplasma groups were signifi?cantly higher than those in LNU group ( P＜0.05). 3) The further analysis showed that the relative abundance of Ruminococcus 1, Ruminobacter and Anaeroplasma had a positive relationship with the host nitrogen utiliza?tion ( P＜0.05). 4) The numbers of Butyrivibrio fibrisolvens, Fibrobacter succinogenes and Ruminococcus_sp._HUN007 in HNU group were significantly higher than those in LNU group ( P＜0.05) , and the numbers of Prevotella ruminicola, Streptococcus bovis, Succinivibrio dextrinisolvens and Ruminococcus flavefaciens had no differences between two groups (P＞0.05). In summary, there are significant differences on the bacterial structure and composition in rumen of goats with different nitrogen utilization.[ Chinese Journal of Animal Nu?trition, 2019, 31(8) :3651?3663]关键词
山羊/氮利用率/瘤胃细菌/高通量测序Key words
goats/nitrogen utilization/rumen bacterial/high⁃throughput sequencing分类
农业科技引用本文复制引用
李琼,王立志,王之盛,薛白,彭全辉..不同氮利用率山羊瘤胃细菌结构与组成的差异[J].动物营养学报,2019,31(8):3651-3663,13.基金项目
四川省学术带头人培养项目(03120244) （03120244）
