动物营养学报2019，Vol.31Issue(8)：3682-3689,8.DOI:10.3969/j.issn.1006⁃267x.2019.08.029
谷氨酰胺二肽对草鱼幼鱼生长、血清生化、免疫指标及肠道组织结构的影响
Effects of Glutamine Dipeptide On Growth, Serum Biochemistry, Immunity Indexes and Intestinal Morphology of Juvenile Grass Carp ( Ctenopharyngodon idellus)
摘要
Abstract
The aim of this experiment was to study the effects of glutamine dipeptide ( Gly?Gln ) on the growth, serum biochemistry, immunity indexes and intestinal morphology of juvenile grass carp. A total of 750 fish with an average body weight of (7.16±0.10) g were selected and divided into 5 groups with 3 replicates per group and 50 fish per replicate. Fish were fed diets containing 0 ( control group) and 0.25%, 0.50%, 0.75% and 1.00% glutamine dipeptide, respectively. The feeding test lasted for 8 weeks. The results showed as follows: 1) compared with the control group, the weight gain rate and protein efficiency of juvenile grass carp increased significantly ( P＜0.05) and the feed coefficient decreased significantly ( P＜0.05) in the glutamine dipeptide supplementation groups. Dietary supplementation of 0.25% and 0.50% glutamine dipeptide signifi?cantly increased intestinal lipase and trypsin activities ( P＜0.05).2) Compared with the control group, the ser?um glucose content in 0.75% group significantly increased ( P＜0.05) , and the serum urea nitrogen content in each glutamine dipeptide supplementation group significantly increased ( P＜0.05). The addition of glutamine dipeptide in the feed made the serum triglyceride and cholesterol contents decrease, and the cholesterol content in 0.50% group significantly decreased compared with the control group ( P＜0.05) , serum triglyceride content in each supplementation group significantly decreased compared with the control group (P＜0.05). The activities of glutamic oxalacetic transaminase and alanine aminotransferase significantly increased when the dosage was more than 0.50%(P＜0.05).3) Compared with the control group, the serum lysozyme activity in 0.50%group increased significantly (P＜0.05). Serum complement 4 content decreased significantly in 0.75% and 1.00% groups ( P＜0.05), while the cortisol content increased significantly (P＜0.05).The serum glutathione content and total antioxi?dant capacity significantly increased in each supplementation group ( P＜0.05). 4) Compared with the control group, the intestinal crypt depth significantly reduced in 0.5% group ( P＜0.05), the intestinal mucosal thickness significantly increased in the 0.75% and 1.00% groups (P＜0.05), and the number of goblet cells significantly in?creased in the 0.50% and 1.00% groups (P＜0.05). In summary, the addition of 0.50% glutamine dipeptide to the feed can promote nutrient metabolism, immunity and intestinal development of grass carp juveniles, thereby pro?moting growth.[Chinese Journal of Animal Nutrition, 2019, 31(8):3682?3689]关键词
谷氨酰胺二肽/草鱼幼鱼/生长性能/免疫/肠道形态Key words
glutamine dipeptide/juvenile grass carp/growth performance/immunity/intestinal morphology分类
农业科技引用本文复制引用
吴桐强,钟蕾,刘庄鹏,胡毅,刘臻,鲁双庆..谷氨酰胺二肽对草鱼幼鱼生长、血清生化、免疫指标及肠道组织结构的影响[J].动物营养学报,2019,31(8):3682-3689,8.基金项目
国家自然科学基金项目(31272675) （31272675）
湖南省科技重大专项(2017NK1030) （2017NK1030）
