动物营养学报2019，Vol.31Issue(8)：3830-3842,13.DOI:10.3969/j.issn.1006⁃267x.2019.08.045
超声波辅助单宁酶法提取五倍子没食子酸及其抗氧化作用研究
Study on Gallic Acid from Galla chinensis by Ultrasonic?Assisted Tannin Enzymatic Method and Its Antioxygenation
摘要
Abstract
This study was conducted to explore the optimum extraction process conditions of gallic acid from Galla chinensist by ultrasonic?assisted tannin enzymatic method and its antioxygenation. A single factor design was adopted in the experiment, to study the effects of tannase enzyme addition amount, enzymolysis tempera?ture, enzymolysis time, pH, ultrasonic power on yield of gallic acid, and the response surface method was used to optimize the extraction process condition of gallic acid from Galla chinensis. Forty healthy Kunming mice ( half male and half female) with the body weight ( BW) of (20±2) g were selected and divided into 4 groups with 10 mice per group. Mice in the blank control group were gavage 10 g BW 0.1 mL normal saline, and mice in the experimental groups were gavage 50 ( low dose group ) , 75 ( medium dose group ) and 100 mg／kg BW ( high dose group) gallic acid solution of the same volume, respectively, one times a day, and the experiment last for 21 days. The results showed that the optimum extraction process conditions of gallic acid were as follows: the tannase enzyme addition amount was 11 U／g, the enzymolysis temperature was 45 ℃,the enzymolysis time was 4.8 h, the pH was 6 and the ultrasonic power was 380 W. Under the above conditions, the yield of gallic acid was ( 64.73±1.61)%. The purity of gallic acid was (99.01±2.47)% which puritied by crystallization method and ion exchange resin method. On the mass concentration of 60 μg／mL, the reducing force was 0.85±0.04, and the scavenging rates of 1,1?diphenyl?2?picrylhydrazine radical (DPPH·) and superoxide anion radical ( O－2·) were ( 92.11 ±2.38)% and ( 98.62 ± 2.75)%, respectively. Compared with the blank control group, the activities of superoxide dismutase ( SOD) , catalase ( CAT) and glutathione peroxidase ( GSH?Px) in liver of medium and high dose groups were significantly increased ( P＜0.05 and P＜0.01) , and the liver malondialdehyde ( MDA) content of low, medium and high dose groups was significantly decreased ( P＜0.05 and P＜0.01). Therefore, the ultrasonic?assisted tannin enzymatic method can improve the yield of gallic acid, the gallic acid can scavenge free radical effectively and has good antioxygenation.[ Chinese Journal of Animal Nutrition, 2019, 31(8) :3830?3842]关键词
五倍子/没食子酸/超声波/单宁酶/响应面法/抗氧化Key words
Galla chinensis/gallic acid/ultrasonic/tannase enzyme/response surface method/antioxidant分类
农业科技引用本文复制引用
李振,李萍..超声波辅助单宁酶法提取五倍子没食子酸及其抗氧化作用研究[J].动物营养学报,2019,31(8):3830-3842,13.基金项目
山东省自然科学基金项目( ZR2015PH029) （ ZR2015PH029）
临沂大学博士科研启动基金项目( LYDX2016BS082) （ LYDX2016BS082）
