动物营养学报2019，Vol.31Issue(8)：3914-3920,7.DOI:10.3969/j.issn.1006⁃267x.2019.08.054
紫锥菊根中菊苣酸的提取分离及其分子印迹聚合物的制备
Isolation and Purification of Chicoric Acid from Echinacea purpurea Root and Its Molecularly Imprinted Polymer Preparation
摘要
Abstract
Chicoric acid is one active phenolic acid component of natural herbal medicine Echinacea purpurea, and this study set out to investigate the new isolation and purification method of chicoric acid from Echinacea purpurea root. The chicoric acid monomer was isolated and purified by the sephadex gel LH?20 column which eluted by 80%, 60%, 40% methanol after extracted by ultrasonic extraction from Echinacea purpurea root powder. For the preparation of molecularly imprinted polymer of chicoric acid, the purified chicoric acid mono?mer above was used as the template molecule with the 4?vinylpyridine and ethylene glycol dimethacrylate to be functional monomer and crosslinker, respectively, and the Echinacea purpurea root powder was enrichment purified by the molecularly imprinted polymer. The results showed that after sephadex gel LH?20 column sepa?rated and purified, the chicoric acid content was 90.22% by high performance liquid chromatography. After en?richment purified by the molecularly imprinted polymer of Echinacea purpurea root powder, the cichoric acid and caffeic acid could simultaneous enrichment which was with the same mother nucleus structure. In conclu?sion, this method can used for the isolation and purification of chicoric acid from echinacea purpurea root and its molecularly imprinted polymer preparation.[ Chinese Journal of Animal Nutrition, 2019, 31 ( 8 ) :3914?3920]关键词
菊苣酸/分子印迹聚合物/提取/纯化/高效液相色谱Key words
chicoric acid/molecularly imprinted polymer/extraction/purification/HPLC分类
农业科技引用本文复制引用
张虎,王庚南,王建平,马雨红,苏铭,刘聚祥..紫锥菊根中菊苣酸的提取分离及其分子印迹聚合物的制备[J].动物营养学报,2019,31(8):3914-3920,7.基金项目
河北省自然基金青年基金项目( C2016204039 ) （ C2016204039 ）
河北省高等学校科学技术研究项目( QN2018170 ) （ QN2018170 ）
河北省蛋鸡体系( HBCT2018150210) （ HBCT2018150210）
