中国神经再生研究（英文版）Issue(1)：152-161,10.DOI:10.4103/1673-5374.264462
Skeletal muscle-derived cells repair peripheral nerve defects in mice
摘要
Abstract
Skeletal muscle-derived cells have strong secretory function, while skeletal muscle-derived stem cells, which are included in muscle-de-rived cells, can differentiate into Schwann cell-like cells and other cell types. However, the effect of muscle-derived cells on peripheral nerve defects has not been reported. In this study, 5-mm-long nerve defects were created in the right sciatic nerves of mice to construct a peripheral nerve defect model. Adult female C57BL/6 mice were randomly divided into four groups. For the muscle-derived cell group, muscle-derived cells were injected into the catheter after the cut nerve ends were bridged with a polyurethane catheter. For external oblique muscle-fabricated nerve conduit and polyurethane groups, an external oblique muscle-fabricated nerve conduit or polyurethane catheter was used to bridge the cut nerve ends, respectively. For the sham group, the sciatic nerves on the right side were separated but not excised. At 8 and 12 weeks post-surgery, distributions of axons and myelin sheaths were observed, and the nerve diameter was calculated using immunofluorescence staining. The number, diameter, and thickness of myelinated nerve fibers were detected by toluidine blue staining and transmission electron microscopy. Muscle fiber area ratios were calculated by Masson’s trichrome staining of gastrocnemius muscle sec-tions. Sciatic functional index was recorded using walking footprint analysis at 4, 8, and 12 weeks after operation. The results showed that, at 8 and 12 weeks after surgery, myelin sheaths and axons of regenerating nerves were evenly distributed in the muscle-derived cell group. The number, diameter, and myelin sheath thickness of myelinated nerve fibers, as well as gastrocnemius muscle wet weight and muscle area ratio, were significantly higher in the muscle-derived cell group compared with the polyurethane group. At 4, 8, and 12 weeks post-surgery, sciatic functional index was notably increased in the muscle-derived cell group compared with the polyurethane group. These criteria of the muscle-derived cell group were not significantly different from the external oblique muscle-fabricated nerve conduit group. Collective-ly, these data suggest that muscle-derived cells effectively accelerated peripheral nerve regeneration. This study was approved by the Animal Ethics Committee of Plastic Surgery Hospital, Chinese Academy of Medical Sciences (approval No. 040) on September 28, 2016.关键词
Key Words: muscle nerve conduit/ myokine/ nerve regeneration/ nerve repair/ peripheral nerve regeneration/ polyurethane catheter/ seed cells/skeletal muscle/ skeletal muscle-derived cells/ tissue-engineered nerveKey words
Key Words: muscle nerve conduit/ myokine/ nerve regeneration/ nerve repair/ peripheral nerve regeneration/ polyurethane catheter/ seed cells/skeletal muscle/ skeletal muscle-derived cells/ tissue-engineered nerve分类
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Zi-Xiang Chen,Hai-Bin Lu,Xiao-Lei Jin,Wei-Feng Feng,Xiao-Nan Yang,Zuo-Liang Qi..Skeletal muscle-derived cells repair peripheral nerve defects in mice[J].中国神经再生研究（英文版）,2020,(1):152-161,10.基金项目
This study was financially supported by the National Natural Science Foundation of China, No. 81671908 (to ZLQ) and No. 81571921 (to XNY) （to ZLQ）
the Fundamental Research Fund for the Central Universities of China, No. 2016ZX310197 (to ZLQ) （to ZLQ）
the Union Youth Science & Research Foundation of China, No. 3332015155 (to XNY) （to XNY）
the Science Fund of Plastic Surgery Hospital, Chinese Academy of Medical Sciences, and Peking Union Medical College of China, No. Q2015013 (to XNY). （to XNY）
