动物营养学报2019，Vol.31Issue(9)：4061-4069,9.DOI:10.3969／j.issn.1006⁃267x.2019.09.018
瘤胃微生物对蛋氨酸的利用及微生物氮流转特征研究
Utilization of Methionine by Rumen Microorganisms and Characteristics of Microbial Nitrogen Transfer
摘要
Abstract
The aim of this study was to investigate the utilization of methionine ( Met) by rumen microorgan?isms and effects of Met on microbial nitrogen transfer. Corn?soybean meal concentrate and ( 0.5 g) and corn?straw diet ( 0.5 g) were used for fermentation substrates, the study was conducted as one?factor block experi?mental design, and set up test group and control group. The test group was supplemented with 0.33 μmol iso?tope labeling 13 C methionine ( 13 C?Met,addition of 0.18 mL solution to in vitro culture substrate) , and the con?trol group was supplemented with equivalent distilled water, to explore the effects of Met on in vitro fermenta?tion pH, the contents of ammonia nitrogen ( NH3?N) , volatile fatty acid ( VFA) , microbial nitrogen and mi?crobial Met and the abundance of 13C in microbial. The results showed as follows: 1) adding Met could signifi?cantly increase the pH of fermentation liquid ( P＜0.01) ; 2) adding Met could significantly increase the abun?dance of 13 C in microorganisms ( P＜0.01 ) ; 3 ) adding Met had no significant effects on the contents of NH3?N, VFA, microbial nitrogen and microbial Met ( P＞0.05). Therefore, it can be inferred that rumen mi?croorganisms can effectively utilize Met, Met has some influence on fermentation characteristics, but do not af?fect the flow of microbial nitrogen.[ Chinese Journal of Animal Nutrition, 2019, 31(9) :4061?4069]关键词
蛋氨酸/13 C丰度/发酵特性/微生物氮Key words
Met/δ13 C/fermentation characteristics/microbial nitrogen分类
农业科技引用本文复制引用
任傲,谭支良,朱晋佳,陈亮,孔志伟,刘倩,郭乙冰,徐琪翔,周传社,张彬..瘤胃微生物对蛋氨酸的利用及微生物氮流转特征研究[J].动物营养学报,2019,31(9):4061-4069,9.基金项目
国家自然科学基金面上项目——瘤胃细菌蛋氨酸与赖氨酸的合成与分解代谢路径及调控机理(31772632) （31772632）
