动物营养学报2019，Vol.31Issue(9)：4186-4193,8.DOI:10.3969／j.issn.1006⁃267x.2019.09.031
低磷浓度下原代培养肉鸡鸡胚十二指肠上皮细胞对磷吸收及相关转运载体表达的研究
Phosphorus Absorption and Related Transporter Expression under Low Phosphorus Concentrations in Primarily Cultured Duodenal Epithelial Cells of Broiler Embryos
摘要
Abstract
The aim of the study was to investigate the phosphorus absorption and related transporter expression under low phosphorus concentrations in primarily cultured duodenal epithelial cells of broiler embryos. A single?factor completely randomized design was used in this experiment, the cells were randomly assigned to 5 groups with 6 replicates per group, and then treated with 0 ( control group) , 0.5, 1.0, 2.0 and 4.0 mmol／L phos?phorus for 87 min, respectively. The results showed that different phosphorus concentrations had significant effect on phosphorus absorption percentage in duodenal epithelial cells ( P＜0.05) , and the phosphorus absorp?tion percentage was linear or quadratic increased with phosphorus concentration increased ( P＜0.05 ). Com?pared with the control group, the mRNA relative expressions of typeⅡb sodium?phosphate cotransporter ( Na?Pi?Ⅱb) and inorganic phosphate transporter 1 ( PiT?1) in duodenal epithelial cells of 4.0 mmol／L group were significantly decreased ( P＜0.05 ) , but the mRNA relative expression of inorganic phosphate transporter 2 ( PiT?2) in duodenal epithelial cells of 4.0 mmol／L group had no significant change ( P＞0.05). Different phosphorus concentrations had no significant effects on the protein expressions of NaPi?Ⅱb, PiT?1 and PiT?2 in duodenal epithelial cells (P＞0.05). In conclusion, with the incubation solution phosphorus concentration in?creased, the cell phosphorus absorption percentage increase; when the incubation solution phosphorus concen?tration is 4.0 mmol／L, the primarily cultured duodenal epithelial cells of broiler embryos can regulate the phos?phorus absorption by reducing the mRNA expressions of NaPi?Ⅱb and PiT?1.[ Chinese Journal of Animal Nu?trition, 2019, 31(9) :4186?4193]关键词
十二指肠上皮细胞/鸡胚/磷/吸收Key words
duodenal epithelial cells/broiler embryos/phosphorus/absorption分类
农业科技引用本文复制引用
吕娜,吕林,廖秀冬,张丽阳,罗绪刚..低磷浓度下原代培养肉鸡鸡胚十二指肠上皮细胞对磷吸收及相关转运载体表达的研究[J].动物营养学报,2019,31(9):4186-4193,8.基金项目
国家自然科学基金重点项目(31630073) （31630073）
国家自然科学基金面上项目(31472116) （31472116）
国家重点研发计划项目(2017YFD0502200) （2017YFD0502200）
国家现代农业产业技术体系岗位专家专项经费( CARS⁃41) （ CARS⁃41）
中国农业科学院科技创新工程专项经费( ASTIP⁃IAS09) （ ASTIP⁃IAS09）
