动物营养学报2019，Vol.31Issue(9)：4242-4250,9.DOI:10.3969／j.issn.1006⁃267x.2019.09.038
补喂复合益生菌制剂对幼犬生长性能、肠道动力及肠道屏障功能的影响
Effects of Supplementary Feeding Complex?Probiotic?Preparation on Growth Performance, Intestinal Motility and Intestinal Barrier Function of Puppies
摘要
Abstract
The purpose of this experiment was to study the effects of supplementary feeding complex?probiotic?preparation on growth performance, intestinal motility and intestinal barrier function of puppies. Forty poodles at the age about 6 weeks were randomly divided into five groups with 2 replicates in each group and 4 poodles in each replicate. Among them, poodles in test group A were fed a basal diet+0.2 g／( kg BW·d) complex?probiotic?preparation; poodles in test group B were fed the basal diet+0.6 g／( kg BW·d) complex?probiotic?preparation; poodles in test group C were fed the basal diet+1.0 g／( kg BW·d) complex?probiotic?prepara?tion; poodles in negative control group were fed the basal diet; and poodles in contrast control group were fed the basal diet+1.0 g／( kg BW·d) G?pet probiotic powder. The experiment lasted for 30 days. After experi?ment to sampling, the contents of gastrointestinal hormones and intestinal barrier function indicators in serum were detected by enzyme?linked immunosorbent assay ( ELISA) method, serum inflammatory factor contents were detected by immunoturbidimetry, and mRNA relative expression levels of related genes in jejunum muco?sa were detected by quantitative real?time fluorescence PCR ( RT?qPCR). The results showed as follows: 1) with the increase of feeding dosage of complex?probiotic?preparation, the final weight and average daily gain were increased, and the diarrhea rate and constipation rate were decreased. The average daily gain of test group C was significantly higher than that of other groups ( P＜0.05) , and the diarrhea rate and constipation rate were significantly lower than those of negative control group ( P＜0.05). 2) With the increase of feeding dosage of complex?probiotic?preparation, the contents of motilin ( MTL) and interleukin?10 ( IL?10) in serum showed an increase trend, while the contents of asoactive intestinal peptide ( VIP ) , D?lactic acid, lipopolysaccharide ( LPS) and tumor necrosis factor?α ( TNF?α) and the activity of diamine oxidase ( DAO) in serum showed a decrease trend. Significant differences in above indices were found between test group C and negative control group ( P＜0.05) , but no significant differences were found between test group C and contrast control group ( P＞0.05). 3) With the increase of feeding dosage of complex?probiotic?preparation, the mRNA relative ex?pression levels of occludin and zonula occluden?1 ( ZO?1) in ileal mucosa were up?regulated, while the mRNA relative expression level of myosin light?chain kinase ( MLCK) was down?regulated. Significant differences in above indices were found between test group C and negative control group ( P＜0.05) , but no significant differ?ences were found between test group C and contrast control group ( P＞0.05). The mRNA relative expression level of ZO?1 in test group C was significantly higher than that of test groups A and B ( P＜0.05). In summa?ry, supplementary feeding complex?probiotic?preparation can enhance the gastrointestinal motility and intestinal barrier function, alleviate the inflammatory stress and improve the growth performance of puppies, and the best supplementary feeding dosage is 1.0 g／kg BW per day.[ Chinese Journal of Animal Nutrition, 2019, 31(9) :4242?4250]关键词
复合益生菌制剂/幼犬/生长性能/肠道动力/肠道屏障功能Key words
complex⁃probiotic⁃preparation/puppy/growth performance/intestinal motility/intestinal barrier function分类
农业科技引用本文复制引用
陆江,朱道仙,卢鹏飞,张东豪,刘静,傅宏庆..补喂复合益生菌制剂对幼犬生长性能、肠道动力及肠道屏障功能的影响[J].动物营养学报,2019,31(9):4242-4250,9.基金项目
江苏省大学生创新创业训练计划项目( 201812806015Y) （ 201812806015Y）
江苏农牧科技职业学院横向课题配套项目( NSFPT201845, NSFPT201842) （ NSFPT201845, NSFPT201842）
