动物营养学报2019，Vol.31Issue(9)：4286-4295,10.DOI:10.3969／j.issn.1006⁃267x.2019.09.043
竹叶黄酮与青蒿提取物对患隐性乳房炎奶牛产奶性能、乳中体细胞数及血清免疫和抗氧化相关指标的影响
Effects of Bamboo Leaf Flavonoids and Artemisia annua Extract on Milk Performance, Milk Somatic Cell Count and Serum Immune and Antioxidant Related Indexes of Dairy Cows with Subclinical Mastitis
摘要
Abstract
This experiment was conducted to investigate the effects of bamboo leaf extract and Artemisia annua extract on milk performance, milk somatic cell count ( SCC) and serum immune and antioxidant related indexes of dairy cows with subclinical mastitis. Eighteen Chinese Holstein cows with subclinical mastitis [ SCC was ( 41.31+0.49)×104 cell／mL] with similar days in milk, similar milk yield, body weight of (590±15) kg, 2 to 4 parities were selected and randomly divided into 3 groups with 6 cows per group for feeding experiment. Cows in control group were a total mixed ration ( TMR) , while cows in bamboo leaf flavones group and Arte?misia annua extract group were also fed TMR, and supplementary fed with bamboo leaf flavones ( 30 g／d) and Artemisia annua extract (60 g／d) , respectively. The feeding experiment was conducted for 35 days, in?cluding pre?test period of 7 days, feeding period of 14 days and post feeding period of 14 days. Blood and milk samples were collected on day 1, 14, 28 to measure milk SCC, milk composition and serum immune?related factor contents and antioxidant indexes, and the milk yield was recorded. The results showed as follows:1) compared with the control group, the milk yield in bamboo leaf flavones group and Artemisia annua extract group was increased by 8.95% and 13.24%( P＞0.05) , respectively, the 4% fat corrected milk ( FCM) yield and milk protein percentage on day 14 were significantly increased ( P＜0.05) , and the milk SCC on day 14 was significantly decreased ( P＜0.05). 2) The contents of interleukin?1β ( IL?1β) and tumor necrosis factor?α ( TNF?α) in serum on day 14 in bamboo leaf flavones group and Artemisia annua extract group were signifi?cantly lower than those in control group ( P＜0.05) ; otherwise, the serum interleukin?2 ( IL?2) content in ser?um in Artemisia annua extract group was significantly higher than that in control group ( P＜0.05). 3) Com?pared with the control group, the serum malondialdehyde ( MDA) content on day 14 was decreased significant?ly ( P＜0.05) , and serum superoxide dismutase ( SOD) activity on day 14 tended to be increased both in bam?boo leaf flavones group and Artemisia annua extract group ( P＝0.06). In conclusion, both bamboo leaf fla?vonoids and Artemisia annua extract can improve the mill performance, enhance the immune function and an?tioxidant capacity of dairy cows, and decrease the milk SCC of dairy cows with subclinical mastitis.[ Chinese Journal of Animal Nutrition, 2019, 31(9) :4286?4295]关键词
竹叶黄酮/青蒿提取物/奶牛/隐性乳房炎/抗氧化能力/免疫功能Key words
bamboo leaf flavonoids/Artemisia annua extract/antioxidant capacity/immune function/dairy cows/subclinical mastitis分类
农业科技引用本文复制引用
侯昆,童津津,楚康康,熊本海,蒋林树..竹叶黄酮与青蒿提取物对患隐性乳房炎奶牛产奶性能、乳中体细胞数及血清免疫和抗氧化相关指标的影响[J].动物营养学报,2019,31(9):4286-4295,10.基金项目
国家自然科学基金项目(31772629,31702302,31802091) （31772629,31702302,31802091）
2019年北京农学院学位与研究生教育改革与发展项目(2019YJS031) （2019YJS031）
