动物营养学报2019，Vol.31Issue(9)：4296-4302,7.DOI:10.3969／j.issn.1006⁃267x.2019.09.044
芦笋皮或象草饲喂肉牛的血清代谢组学研究
Study on Serum Metabolomics of Cattle Fed Asparagus Peel or Napiergrass
摘要
Abstract
This study was conducted to investigate the effects of asparagus peel or napiergrass on serum metab?olites of cattle based on metabonomics technique. Ten healthy Angus beef cattle [ (236.50±34.00) kg of body weight] with similar ages were randomly divided into 2 groups with 5 replicates per group and 1 cattle per rep?licate. Cattle in the two group were fed the total mixed rations ( TMR) with asparagus peel or napiergrass as roughage. The pre?experimental period lasted for 15 days, and the experimental period lasted for 45 days. On the last day of experiment, the blood samples were collected from tail venous before morning feeding, and then serum was isolated, and serum metabolites were detected by gas chromatography?mass spectrometry ( GC?MS) technique, using principal component analysis ( PCA) showed the distribution of metabolites, screened and i?dentified the metabolites, and analysis the metabolic pathways. The results showed that 16 different metabolites were obtained in two groups. Twelve metabolites of asparagus peel group were up?regulated compared with napiergrass group including biuret, β?glutamic acid, cycloleucine and 2?deoxy?D?glucose, etc, but L?cysteine, cumic acid, N?acetyltryptophan and isoleucine were down?regulated, these metabolites were mainly related to 7 pathways including alanine, aspartate and glutamate metabolism, arginine and proline metabolism, valine, leu?cine and isoleucine biosynthesis and citrate cycle, etc. In conclusion, under the conditions of this experiment, feeding cattle with asparagus peel is more beneficial to nutrient metabolism than napiergrass.[ Chinese Journal of Animal Nutrition, 2019, 31(9) :4296?4302]关键词
芦笋皮/象草/肉牛/血清/代谢组学Key words
asparagus peel/napiergrass/cattle/serum/metabolomics分类
农业科技引用本文复制引用
玉霞,王启业,张雪蕾,杨焕胜,高树新,吴端钦..芦笋皮或象草饲喂肉牛的血清代谢组学研究[J].动物营养学报,2019,31(9):4296-4302,7.基金项目
湖南省科技重大专项(2017NK1020) （2017NK1020）
湖南省重点研发计划(2016NK2170) （2016NK2170）
