动物营养学报2019，Vol.31Issue(10)：4509-4518,10.DOI:10.3969／j.issn.1006⁃267x.2019.10.014
大约克猪肠道不同部位的菌群结构和短链脂肪酸含量研究
Study on Bacterial Community Structure and Short Chain Fatty Acid Content in Different Parts of Intestines of Yorkshire Pigs
摘要
Abstract
This experiment was to study the similarities and differences of bacterial community structure and short chain fatty acid content in different parts of intestines of Yorkshire pigs. Three Yorkshire pigs of 180 days old weighing (121.00±2.89) kg were selected, and the contents in duodenum, jejunum, ileum, cecum and colon were collected. Then the microbial genomic DNA was extracted and sequenced by Illumina Hiseq sequen?cing platform with high throughput to analyze the flora structure, and the short chain fatty acid ( SCFA) con?tent was determined. The results indicated that total bacteria, Firmicutes and Bacteroidetes were significantly in?creased from duodenum to colon ( P＜0.05). The cecum and colon showed richer bacteria flora species compo?sition, higher diversity and uniformity compared with duodenum, jejunum and ileum ( P＜0.05). Firmicutes dominated the intestinal flora, followed by Proteobacteria in duodenum, jejunum and ileum and Bacteroidetes in cecum and colon at the phylum level. Lactobacillus, SMB53, Clostridium in Firmicutes and Vibrio in Pro?teobacteria were the most abundant genera in duodenum, jejunum and ileum, and Lactobacillus, SMB53, Streptococcus in Firmicutes and Prevotella in Bacteroidetes were the most abundant genera in cecum and colon at the genus level. Compared with duodenum, jejunum and ileum, the relative abundance of Lactobacillus, Vibrio, Staphylococcus and Acinetobacter in cecum and colon was decreased significantly ( P＜0.05) , while the relative abundance of bacteria that produce SCFA such as Ruminococus, Bacteroides, Prevotella, Copro?coccus, Oscillospira, Roseburia, Lachnospira and Faecalibacterium was increased significantly ( P＜0.05). The beta diversity analysis showed that the similar of bacterial community structure in duodenum, jejunum and ileum was relatively high, and the similar of bacterial community structure in cecum and colon was relatively high which was significantly different from that in small intestine ( P＜0.05). Meanwhile, SCFA content in ce?cum and colon was significantly higher than that in small intestine ( P＜0.05). Therefore, the bacterial commu?nity structure in the small intestine and large intestine of Yorkshire pigs is significantly different. In the small in?testine, the main bacterial floras are Firmicutes and Proteobacteria, while in the large intestine, Firmicutes and Bacteroidetes are dominant. Large intestine has high relative abundance of typical SCFA?producing bacteria such as Bacteroides, Prevotella, Weissella, Roseburia, Treponema and Ruminococcus and high SCFA con?tent, and there is a significant positive correlation between them.[ Chinese Journal of Animal Nutrition, 2019, 31(10) :4509?4518]关键词
大约克猪/肠道菌群/高通量测序/短链脂肪酸Key words
Yorkshire pig/intestinal microflora/high throughput sequencing/short chain fatty acid分类
农业科技引用本文复制引用
徐娥,杨华,刘秀婷,任敏敏,申露露,吕文涛,肖英平..大约克猪肠道不同部位的菌群结构和短链脂肪酸含量研究[J].动物营养学报,2019,31(10):4509-4518,10.基金项目
贵州大学博士基金(2012021) （2012021）
金华市科学技术局农业类重点项目(2016⁃2⁃001) （2016⁃2⁃001）
贵州省中药现代化科技产业研究开发专项项目(20135029) （20135029）
