动物营养学报2019，Vol.31Issue(10)：4590-4601,12.DOI:10.3969／j.issn.1006⁃267x.2019.10.023
N－氨甲酰谷氨酸对花鲈生长性能、形体指标和血浆生化指标的影响
Effects of N?Carbamylglutamate on Growth Performance, Morphometric Parameters and Plasma Biochemical Parameters of Japanese Seabass ( Lateolabrax japonocus)
摘要
Abstract
A 10?week growth trial was to determine the effects of different supplemental levels of N?carbamyl?glutamate ( NCG) in the diet on growth performance, morphometric parameters and plasma biochemical pa?rameters of Japanese seabass ( Lateolabrax japonocus) , and to determine the optimum requirement of NCG for Japanese seabass. Six experimental diets were designed with NCG supplement levels of 0 ( N0) , 120 ( N120) , 240 ( N240) , 360 ( N360) , 480 ( N480) and 720 mg／kg ( N720) , respectively. Each diet was fed to 6 rep?licates with 30 Japanese seabass [ initial body weight＝(11.67±0.02) g] in each replicate. The results showed that no significant differences in final body weight ( FBW ) , weight gain rate ( WGR ) , fat retention ratio ( FRR) , special growth rate ( SGR) , viscerasomatic index ( VSI) and hepatosomatic index ( HSI) were ob?served among groups ( P＞0.05). The N240 group had the highest protein retention ratio ( PRR) and the lowest abdominal fat percentage ( AFP) , and the PRR was significantly higher than that in the groups except N360 group ( P＜0.05) , while the AFP was significantly lower than that in the N480 group ( P＜0.05). Hepatic fat content was the lowest in the N360 group, which was significantly lower than that in the N0 and N120 groups ( P＜0.05). There were no significant differences in plasma glucose ( GLU) , total cholesterol ( TC) , low den?sity lipoprotein cholesterin ( LDL?C) contents and aspartate aminotransferase ( AST) , alanine aminotransferase ( ALT) activities ( P＞0.05). Plasma urea nitrogen ( UN) content in the N120 group was significantly lower than that in the N0, N480 and N720 groups ( P＜0.05). Plasma triglyceride ( TG) content in the N120 group was significantly lower than that in the N0 and N360 groups ( P＜0.05). Plasma high density lipoprotein cho?lesterin ( HDL?C) content in the N240 group was significantly higher than that in the N360 and N720 groups ( P＜0.05). Plasma nitric oxide ( NO) content was the highest in the N360 group which was significantly high?er than that in the N480 group ( P＜0.05). The results indicate that appropriate dietary NCG supplementation can promote the protein retention, decrease the fat accumulation in liver and abdomen, and improve the metab?olism of protein and lipid in Japanese seabass. According to the broken?line models, taking PRR and AFP as e?valuation indexes, the highest PRR can be obtained when dietary NCG supplemental level is 219.2 mg／kg, and the lowest AFP can be obtained when dietary NCG supplemental level is 214.3 mg／kg. By comprehensive anal?ysis, optimal dietary NCG supplemental level in the diet of Japanese seabass is 214.3 to 219.2 mg／kg.[ Chi?nese Journal of Animal Nutrition, 2019, 31(10) :4590?4601]关键词
花鲈/N－氨甲酰谷氨酸/适宜添加水平/蛋白质沉积率/腹脂率Key words
Japanese seabass ( Lateolabrax japonocus) /N⁃carbamylglutamate/optimal supplemental level/protein retention ratio/abdominal fat percentage分类
农业科技引用本文复制引用
黄皓琰,郁欢欢,梁晓芳,郑银桦,王春平,吴秀峰,陈沛,薛敏..N－氨甲酰谷氨酸对花鲈生长性能、形体指标和血浆生化指标的影响[J].动物营养学报,2019,31(10):4590-4601,12.基金项目
国家重点研发计划项目(2018YFD0900400,2016YFF0201800) （2018YFD0900400,2016YFF0201800）
国家自然科学基金项目(31372539,31572631) （31372539,31572631）
国家重点基础研究发展计划项目( 2014CB138600 ) （ 2014CB138600 ）
北京市现代农业产业技术体系( SCGWZJ 20171103⁃1 ) （ SCGWZJ 20171103⁃1 ）
中国农业科学院基本科研业务费(1610382016010) （1610382016010）
中国农业科学院创新工程项目(CAAS⁃ASTIP⁃2017⁃FRI⁃08) （CAAS⁃ASTIP⁃2017⁃FRI⁃08）
