动物营养学报2019，Vol.31Issue(10)：4647-4658,12.DOI:10.3969／j.issn.1006⁃267x.2019.10.029
丁酸梭菌对2种遗传背景仔猪肠道屏障功能的影响及其分子机制
Effects of Clostridium butyricum on Intestinal Barrier Function of Piglets with Two Genetic Backgrounds and Its Molecular Mechanism
摘要
Abstract
This study was aimed to investigate the effects of Clostridium butyricum on the health status, intesti?nal development, intestinal barrier function, serum cytokine contents and mucosal immunity key protein expres?sion of weaned piglets with two genetic backgrounds. A 2×2 factors design was used in this experiment, 12" Northeastern indigenous×Berkshire" black piglets with similar body weight and (21±2) days of age were se?lected and divided into 2 groups ( control group and experimental group) , randomly, and 12 " Duroc×Land?race×Yorkshire" white piglets with similar body weight and (21±2) days of age were selected and divided into 2 groups ( control group and experimental group) , randomly. Each group was divided into 6 replicates and each pig was fed in single column. Weaned piglets in control groups were fed basal diets, and those in experi?mental groups were fed the basal diets supplemented with 5×1011 CFU／kg Clostridium butyricum preparation for 14 days after 3 days adaption. The results showed as follows: 1) compared with the each control group, the platelet counts of piglets in the two experimental groups were both increased significantly ( P＜0.05) , the villus length to crypt depth ratios in both jejunum and ileum of the two kinds of piglets were increased significantly ( P＜0.05) , the serum D?lactic contents of the two kinds of weaned piglets were decreased significantly ( P＜0.05) , the claudin?1 mRNA relative expression level in jejunum, and the mRNA relative expression levels of claudin?1, zonula occludens?1 ( ZO?1) and occludin in ileum of piglets in binary hybridization experimental group were all increased significantly ( P＜0.05) , the mRNA relative expression levels of ZO?1 and occludin in jejnum of piglets in ternary hybridization experimental group were both increased significantly ( P＜0.05) , the mRNA relative expression levels of Toll?like receptor 2 ( TLR2 ) , Toll?like receptor 4 ( TLR4 ) and myeloid differentiation factor 88 ( MyD88) in jejunum mucosa and TLR2, TLR4 and B?cell lymphoma 3 protein ( Bcl3) in ileum mucosa of piglets in binary hybridization experimental group were increased significantly ( P＜0.05) , and the mRNA relative expression levels of TLR2 in jejunum mucosa and TLR2, TLR4, MyD88, Toll?interac?ting protein ( Tollip) and Bcl3 in ileum mucosa of piglets in ternary hybridization experimental group were in?creased significantly ( P＜0.05). 2) Compared with the ternary hybridization experimental group, the mRNA relative expression levels of claudin?1, TLR4 and MyD88 in jejunum and claudin?1, ZO?1 and occludin in ile?um of piglets in binary hybridization experimental group were increased significantly ( P＜0.05) , but the mR?NA relative expression levels of TLR2, TLR4, Tollip and Bcl3 in ileum mucosa of piglets in binary across ex?perimental group were decreased significantly ( P＜0.05). In conclusion, Clostridium butyricum can improve intestinal development of piglets, activate TLR2／4 signaling pathway in intestinal mucosa of two genetic back?ground piglets, promote the expression of key negative feedback factors in the pathway, increase the expression of tight junction proteins, decrease the intestinal mucosal permeability, improve intestinal barrier function, and there are certain differences in the regulation of intestinal barrier function of the two genetic background weaned piglets.[ Chinese Journal of Animal Nutrition, 2019, 31(10) :4647?4658]关键词
丁酸梭菌/二元杂交/三元杂交/断奶仔猪/Toll样受体2/Toll样受体4Key words
Clostridium butyricum/binary hybridization/ternary hybridization/weaned piglets/Toll⁃like re⁃ceptor 2/Toll⁃like receptor 4分类
农业科技引用本文复制引用
李海花,李玉鹏,王柳懿,王文杰,乔家运..丁酸梭菌对2种遗传背景仔猪肠道屏障功能的影响及其分子机制[J].动物营养学报,2019,31(10):4647-4658,12.基金项目
国家自然科学基金项目(31702147) （31702147）
天津市农业科学院青年科研人员创新研究与实验项目(编号:2018008) （编号:2018008）
天津市""131"创新型人才团队(20180338) （20180338）
