动物营养学报2019，Vol.31Issue(10)：4675-4683,9.DOI:10.3969／j.issn.1006⁃267x.2019.10.032
脱氧雪腐镰刀菌烯醇诱导仔猪海马神经细胞凋亡的线粒体通路研究
Deoxynivalenol Induces Apoptosis in Piglet Hippocampal Nerve Cells via Mitochondrial Pathway
摘要
Abstract
The aim of this experiment was to investigate the mechanism of neurotoxicity of deoxynivalenol ( DON) on piglet hippocampal nerve cells. Piglet hippocampal nerve cells were selected and cultured to loga?rithmic phase, and exposed to 0, 250, 500, 1 000 ng／mL DON and 1 000 ng／mL DON+20 μmol／L cystei?nyl aspartate?specific protease ( Caspase)?8 inhibitor ( FMK) for 24 h, respectively. After 24 h culture, cell apoptosis ratio, reactive oxygen ( ROS) level and mitochondrial membrane potential were determined by flow cytometry, and the main apoptosis genes in mitochondrial pathway including B?cell lymphoma?2 ( Bcl?2) , Bcl?2 associated X protein ( Bax ) , BH3 interacting domain death agonist ( Bid ) , Caspase?2, Caspase?3 and Caspase?9 mRNA expression levels were determined by fluorescence quantitative PCR ( qRT?PCR). The re?sults showed as follows: 1 ) compared with the blank group, the cell apoptosis ratio in 250, 500 and 1 000 ng／mL DON groups was significantly increased ( P＜0.05 or P＜0.01) , however, compared with the 1 000 ng／mL DON group, the apoptosis ratio in 1 000 ng／mL DON+FMK group was significantly decreased ( P＜0.01). 2) Compared with the blank group, the ROS level in 250, 500 and 1 000 ng／mL DON groups was significantly increased ( P＜0.01) , while when compared with 1 000 ng／mL DON group, the ROS level in 1 000 ng／mL DON+FMK group was significantly decreased ( P＜0.01 ). 3 ) Compared with the blank group, the mitochondrial membrane potential in 250, 500 and 1 000 ng／mL DON groups was significantly de?creased ( P＜0.01) , but when compared with 1 000 ng／mL DON group, the mitochondrial membrane potential in 1 000 ng／mL DON+FMK group was significantly increased ( P＜0.01). 4) The mRNA expression levels of Bax, Bid, Caspase?2, Caspase?9 and Caspase?3 in 500 and 1 000 ng／mL DON groups were significantly in?creased ( P＜0.05 or P＜0.01) , while the expression level of Bcl?2 mRNA in 500 and 1 000 ng／mL DON groups was significantly decreased when compared with the blank group ( P＜0.01) ; however, compared with 1 000 ng／mL DON group, the expression levels of Bax, Bid, Caspase?2, Caspase?9 and Caspase?3 mRNA in 1 000 ng／mL DON+FMK group were significantly decreased ( P＜0.01) , and the expression level of Bcl?2 mRNA in 1 000 ng／mL DON+FMK group was significantly increased ( P＜0.01). These results suggest that DON induces apoptosis in piglet hippocampal nerve cells through the mitochondrial pathway.[ Chinese Journal of Animal Nutrition, 2019, 31(10) :4675?4683]关键词
脱氧雪腐镰刀菌烯醇/仔猪海马神经细胞/细胞凋亡/线粒体通路Key words
deoxynivalenol/piglet hippocampal nerve cells/cell apoptosis/mitochondrial pathway分类
生物科学引用本文复制引用
朱雷,曹利,申茂玉,陈晓芳,李玉,冯士彬,吴金节,王希春..脱氧雪腐镰刀菌烯醇诱导仔猪海马神经细胞凋亡的线粒体通路研究[J].动物营养学报,2019,31(10):4675-4683,9.基金项目
国家自然科学基金项目(31472250) （31472250）
安徽省生猪产业技术体系(AHCYTX⁃05⁃07) （AHCYTX⁃05⁃07）
