动物营养学报2019，Vol.31Issue(10)：4757-4765,9.DOI:10.3969／j.issn.1006⁃267x.2019.10.041
添加槐花、香蕉花和杭白菊对体外诱导人工瘤胃酸中毒缓解效果的研究
Alleviating Effects of Adding Sophora japonica, Musa nana flower and Chrysanthemum morifolium on Induced Artificial Rumen Acidosis in Vitro
摘要
Abstract
The aim of this experiment was to investigate the alleviating effect of adding Sophora japonica, Musa nana flower and Chrysanthemum morifolium on induced artificial rumen acidosis in vitro, and to provide a theoreti?cal basis for the application of alleviating rumen acidosis in production. The experiment used in vitro batch culture to study the effects of adding Sophora japonica, Musa nana flower and Chrysanthemum morifolium on rumen fer?mentation parameters in substance with high non?fibrous carbohydrates substance [non?fibrous carbohydrates／neu?tral detergent fiber (NFC／NDF) was 2.30] and conventional substance (NFC／NDF was 1.19), respectively. Sin?gle factor design was used in the experiment, the experiment was divided into 4 groups, and each group was re?peated 3 times.Three plants were added 10 mg／mL in rumen fluid, and the control group did not add plant powder. Samples were collected on 3, 6, 9 and 12 h cultured in vitro and fermentation parameters were measured. The re?sults showed as follows: 1) in conventional substance, the pH at each fermentation stage of Musa nana flower group was higher than that of the control group (P＞0.05); in high non?fibrous carbohydrates substance, the pH at each fermentation stage of control group was significantly higher than that of Chrysanthemum morifolium group (P＜0.05).2) In conventional substance, at fermentation 3 and 6 h, the concentration of lactic acid in fermentation fluid in vitro of Musa nana flower group was significantly higher than that of the control group (P＜0.05); at fer?mentation 12 h, the concentration of lactic acid in fermentation fluid in vitro of Sophora japonica group, Musa na?na flower group and Chrysanthemum morifolium group was significantly lower than that of the control group (P＜0.05). In high non?fibrous carbohydrates substance, at fermentation 3 and 6 h, the concentration of lactic acid in fermentation fluid in vitro of Musa nana flower group was significantly higher than that of the control group (P＜0.05); at fermentation 12 h, the concentration of lactic acid in fermentation fluid in vitro of Chrysanthemum morifolium group was significantly lower than that of the control group (P＜0.05).3) At fermentation 12 h, there was no significant difference on concentration of ammonia nitrogen in fermentation fluid in vitro among all groups (P＞0.05), but there were differences in the fermentation process. 4) In conventional substance, at fermentation 12 h, the total volatile fatty acids concentration and propionic acid proportion in fermentation fluid in vitro of So?phora japonica group and Chrysanthemum morifolium group were significantly higher than that of control group and Musa nana flower group (P＜0.05), and the proportions of acetic acid and butyric acid were significantly lower than that of control group and Musa nana flower group (P＜0.05).In high non?fibrous carbohydrates substance, at fermentation 12 h, the concentration of total volatile fatty acids in fermentation fluid in vitro of Chrysanthemum morifolium group were significantly higher than that of control group and Musa nana flower group (P＜0.05), and the proportions of acetic acid, propionic acid and butyric acid had no significant difference with the control group (P＞0.05). In conclusion, Chrysanthemum morifolium can alleviate the accumulation of lactic acid in rumen, and Musa nana flower can balance the rumen pH, therefore Musa nana flower and Chrysanthemum morifolium have po?tential ability to alleviate rumen acidosis.[Chinese Journal of Animal Nutrition, 2019, 31(10):4757?4765]关键词
槐花/香蕉花/杭白菊/瘤胃酸中毒Key words
Sophora japonica/Musa nana flower/Chrysanthemum morifolium/rumen acidosis分类
农业科技引用本文复制引用
范曜天,孙劼,夏光亮,赵芳芳,孙华,王洪荣..添加槐花、香蕉花和杭白菊对体外诱导人工瘤胃酸中毒缓解效果的研究[J].动物营养学报,2019,31(10):4757-4765,9.基金项目
江苏省现代农业(肉羊)产业技术体系( JATS[2018]305) （肉羊）
国家自然科学基金项目(31872988,31572429) （31872988,31572429）
扬州大学高端人才支持计划(2016) （2016）
