动物营养学报2019，Vol.31Issue(10)：4766-4775,10.DOI:10.3969／j.issn.1006⁃267x.2019.10.042
里氏木霉发酵提取黑豆异黄酮及其对雌性大鼠卵巢早期生长反应基因－1表达的影响
Black Bean Isoflavones Extracted by Trichoderma rimannii Fermentation and Its Effects on Ovarian Early Growth Response Gene?1 Expression of Female Rats
摘要
Abstract
The purpose of this study was to investigate the fermentation conditions of black soybean isoflavones extracted by Trichoderma reesei fermentation, and the effects of black soybean isoflavones on expression of early growth response gene?1 ( EGR?1) in ovarian of female rats. The single factor test and orthogonal test were used to study the suitable fermentation conditions of black soybean isoflavones extracted by Trichoderma reesei fermentation. Forty young SD female rats at 49days of age with body weight of (200±20) g were select?ed as the youth group, and forty elderly SD female rats at 12 months of age with body weight of (490±20) g were selected as the elderly group, rats in youth group and elderly group were each divided into 5 groups with 8 rats per group, which were blank group ( NC group) , positive control group ( PC group ) and low ( L group), medium ( M group) , high dose black bean isoflavones groups ( H group). Rats in L, M and H groups were given 100, 200 and 300 mg／( kg BW·d) black bean isoflavone by gavage, and rats in the PC group were given 0.5 mg／( kg BW·d) diethylstilbestrol by gavage, lasted for 30 days. The results showed that the optimal conditions for the black bean isoflavones extracted by Trichoderma reesei fermentation were fermentation time of 48 h, bottled ratio of 1∶10, the ratio of bacterial liquid to soybean powder of 7∶1, and the best extraction rate was about 12.06%. After the treatment with black bean isoflavones, compared with the NC group, the expression levels of EGR?1 protein and mRNA in M, H and PC groups were significantly increased ( P＜0.05 or P＜0.01). Compared between youth group and elderly group, the expression level of EGR?1 pro?tein in L and M groups was significantly difference ( P＜0.01) , and the expression levels of EGR?1 gene in L and H groups was significantly difference ( P＜0.05 or P＜0.01). In conclusion, as a phytoestrogens, black bean isoflavones can increase the expression levels of EGR?1 protein and mRNA at a certain dose, and the effect is similar to estrogen.[ Chinese Journal of Animal Nutrition, 2019, 31(10) :4766?4775]关键词
黑豆异黄酮/里氏木霉/雌性大鼠/早期生长反应基因－1Key words
black bean isoflavones/Trichoderma reesei/female rats/early growth response gene⁃1分类
农业科技引用本文复制引用
初晓丽,丛莎,孔凡秀,肖宇洋,迟晓星..里氏木霉发酵提取黑豆异黄酮及其对雌性大鼠卵巢早期生长反应基因－1表达的影响[J].动物营养学报,2019,31(10):4766-4775,10.基金项目
黑龙江八一农垦大学研究生创新基金( YJSCX2018⁃Y49) （ YJSCX2018⁃Y49）
国家自然科学基金项目(81673170) （81673170）
黑龙江八一农垦大学"三横三纵"科研团队支持计划——杂粮营养与质量安全创新团队项目( TDJH201806) （ TDJH201806）
黑龙江省应用技术研究与开发计划重大项目( "南病北治、北药南用"专项) ( GY2017YD0215) （ "南病北治、北药南用"专项）
