动物营养学报2019，Vol.31Issue(10)：4825-4833,9.DOI:10.3969／j.issn.1006⁃267x.2019.10.048
Enterobacter cloacae Z0206生物纳米单质硒的安全性及其相对生物学效价评定
Assessment of Enterobacter cloacae Z0206 Biogenic Nanoselenium Safety and Relative Bioavailability
摘要
Abstract
This study was conducted to evaluate the safety and relative bioavailability of Enterobacter cloacae Z0206 biogenic nanoselenium ( BNS). Firstly, using twenty specific pathogen free ( SPF) Kunming mouse ( half male and half female, 18 to 22 g) as experimental animals, the acute oral toxicity test was used to evalu?ate the safety of BNS. On this basis, eighty?four 4?week?old Wistar male rats of clean grade were selected and randomly divided into two groups?control group ( 24 rats) and selenium?deficient group ( 60 rats). Rats in the selenium?deficient group were fed a selenium?deficient diet ( selenium content was 0.02 mg／kg) , while rats in the control group was fed a basic diet ( selenium content was 0.15 mg／kg) which supplemented with sodium selenite based on the selenium?deficient diet. After feeding 21 days, six rats in each group were randomly sam?pled, and then the remaining 54 rats in the selenium?deficient group were randomly divided into 3 groups with 6 replicates and 3 rats each replicate, to receive selenium compensation from different sources of selenium for 35 days. Rats in the 3 groups were fed diets supplemented with sodium selenite, yeast selenium and BNS as se?lenium sources based on the selenium?deficient diet, respectively, the selenium content in the 3 diets were all 0.15 mg／kg. The results showed that BNS’s half lethal dose ( LD50) for mice＞90.46 mg／kg BW ( as seleni?um) , which indicated that it had good safety; after 21 days of selenium depletion, the selenium content in liv?er, kidney and muscle and glutathione peroxidase ( GPx) activity in plasma and liver of rats in the selenium?deficient group were significantly reduced compared with control ( P＜0.01) ; after 35 d post selenium supple?mentation, compared with sodium selenite, BNS could significantly increase the average daily gain and the se?lenium content in renal and muscle ( P＜0.05) , had a tendency of increase GPx activity in plasma and liver ( P＞0.05) , and it slightly better than yeast selenium. Relative to sodium selenite, with the GPx activity in plas?ma and liver and the selenium content in liver, kidney and muscle as evaluation indexes, the relative bioavail?abilities of BNS were 112.2%, 114.6%, 102.0%, 155.3% and 143.2% and the relative bioavailability of yeast selenium were 101.1%, 101.7%, 103.4%, 149.4% and 110.6%, respectively. Collectively, BNS has better safety; compared with sodium selenite, BNS has evident advantages on improving feed intake, selenium deposition in tissues and selenium containing antioxidant enzyme activity of rats, and its relative bioavailability better than sodium selenite and yeast selenium.[ Chinese Journal of Animal Nutrition, 2019, 31(10) :4825?4833]关键词
Enterobacter cloacae Z0206生物纳米单质硒/相对生物学效价/抗氧化/硒沉积/安全性Key words
Enterobacter cloacae Z0206 biogenic nanoselenium/relative bioavailability/antioxidant/seleni⁃um deposition/safety分类
农业科技引用本文复制引用
胡喻涵,李笑笑,黄向韵,王凤芹,汪以真,路则庆..Enterobacter cloacae Z0206生物纳米单质硒的安全性及其相对生物学效价评定[J].动物营养学报,2019,31(10):4825-4833,9.基金项目
国家现代生猪产业技术体系岗位科学家项目(CARS⁃35) （CARS⁃35）
浙江省重点研发计划项目(2015C02022) （2015C02022）
