烟草科技2019，Vol.52Issue(9)：17-24,8.
氯化钙对酸雨胁迫下烤烟叶片表观特征和叶绿素荧光特性的影响
Effects of CaCl2 on phenotype and chlorophyll fluorescence characteristics of flue-cured tobacco under acid rain stress
摘要
Abstract
To reveal the effects of acid rain and exogenous CaCl2 on the phenotype characteristics and chlorophyll fluorescence of flue-cured tobacco leaves, tobacco seedlings (cv. Longjiang911) were planted in artificial climatic incubator and the treatment of simulated acid rain was conducted by a sprayer in greenhouse. The results showed that comparing with the control (CK), artificial severe acid rain (pH ≤ 3.5) resulted in yellow and chlorotic spots on leaves and lower absorption ability to light energy during tobacco seedlings. The treatment also destroyed the function of PSⅡ acceptor-side and inhibited electron transport. In the PSⅡ system of tobacco leaves, the absorption photochemical energy (ABS/RC), the dissipation photochemical energy (DIo/RC) and the dissipation energy from QA oxidation-reduction reaction (TRo/RC) increased significantly, which indicated that acid rain stress affected the activity of PSⅡ reaction center. The proportions of PSⅡ quantum yield ( Φ PSⅡ) and basic fluorescence and heat dissipation quantum yields ( Φ f,D) decreased. However, the proportion of heat dissipation quantum yield ( Φ NF) of inactive PSⅡ reaction center significantly increased, which suggested that severe acid rain reduced the kinetics of ATP synthesis in chloroplasts, limited the photoprotective mechanism of xanthophyll cycle and greatly increased the proportion of inactivation PSⅡreaction centers. Exogenous CaCl2 (10 mmol·L-1 CaCl2 solution sprayed on tobacco leaves) alleviated the damage to photosynthetic system caused by the acid rain treatment.关键词
烤烟/模拟酸雨胁迫/表观特征/叶绿素/荧光特性/氯化钙Key words
Flue-cured tobacco/Simulated acid rain stress/Phenotype characteristic/Chlorophyll/Fluorescence characteristic/Calcium chloride分类
农业科技引用本文复制引用
徐宗艺,陈泉印,米良,黄雨萌,王月,孙广玉..氯化钙对酸雨胁迫下烤烟叶片表观特征和叶绿素荧光特性的影响[J].烟草科技,2019,52(9):17-24,8.基金项目
东北林业大学大学生创新训练项目"烟草光合特性对酸雨胁迫的响应机制及外源钙的缓解作用"(201810225139) （201810225139）
国家自然科学基金项目"植物叶片GSNOR代谢途径在吸收大气NO2中的功能解析及其分子调控机理"(31870373). （31870373）
