烟草科技2019，Vol.52Issue(9)：25-30,6.
玉溪、许昌烤烟烟叶中淀粉酶基因的表达及酶活性分析
Gene expression and enzyme activities of amylase genes in flue-cured tobacco leaves from Xuchang and Yuxi
摘要
Abstract
In order to identify possible causes behind the differences of sugar contents between cured tobacco leaves from Henan and Yunnan, starch degradation-related genes were identified on the basis of gene array data of middle tobacco leaves grown in Xuchang and Yuxi at mature stage. The gene array results were verified by qPCR and enzyme activity, and the contents of starch and total sugar were detected. The results showed that there were four starch degradation-related genes: two α-amylase genes and two β-amylase genes. The expressions of four amylase genes and the activities of α- and β-amylases were higher in Yuxi leaves than in Xuchang leaves. The starch contents in middle tobacco leaves from Yuxi and Xuchang at mature stage were 33.00% and 35.00% , respectively. And there was no obvious difference of starch content between Yuxi and Xuchang leaves. The total sugar content in Yuxi tobacco leaves (2.10% ) before flue-curing was lower than that in Xuchang (3.20% ). However, the total sugar content in cured Yuxi leaves (36.46% ) was higher than that in cured Xuchang leaves (20.60%). The results indicated that amylase was a key influencing factor of the total sugar content in cured leaves, while the starch accumulation in tobacco leaves before flue-curing was not.关键词
烟草/淀粉酶/活性/基因表达Key words
Tobacco/Amylase/Activity/Gene expression分类
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翟妞,周会娜,徐国云,郑庆霞,何声宝,张慧,刘萍萍,许亚龙,陈千思,王晨..玉溪、许昌烤烟烟叶中淀粉酶基因的表达及酶活性分析[J].烟草科技,2019,52(9):25-30,6.基金项目
烟草基因组重大专项项目"烟草基因功能元件全景图构建及关键基因挖掘与调控节点研究"[110201601033(JY-07)]和"典型香型烟草品质形成的代谢特征研究"[110201401012(JY-12)]. （JY-07）
