烟草科技2019，Vol.52Issue(9)：55-63,9.
基质匹配溶剂校准-GC-MS/MS法测定卷烟主流烟气B[a]P
Determination of benzo[a]pyrene in mainstream cigarette smoke using matrix matched solvent calibration-gas chromatography/tandem mass spectrometry method
摘要
Abstract
A novel method to quantify trace benzo[a]pyrene (B[a]P) in mainstream cigarette smoke by matrix matched solvent calibration-gas chromatography/tandem mass spectrometry (MMSC-GC-MS/MS) was developed. The chromatography was conducted by injecting the extract directly after extracting Cambridge filters with oscillating extraction method. The preparation process of matrix matched solvent, injection pattern and MS parameters were investigated, and sample preparation steps and target’s extraction pattern were optimized. The results showed that: 1) Under the optimized conditions, the calibration curve was linear (R2=0.999 6) in the target concentration range of 1.04-20.8 ng/mL with the limits of detection and quantitation at 0.14 and 0.46 ng/cig. respectively, which were lower than the reported values of the current Chinese National Standard method. The spiked recoveries of different samples ranged from 94.62% to 104.81% with intra- and inter-day relative standard deviations (RSDs) at 3.13%-3.80% and 5.41%-6.94% , respectively. 2) No significant deviation was observed between the results of B[a]P in mainstream smoke of 18 slim and conventional cigarettes with different labeled tar levels determined by the proposed MMSC-GC-MS/MS method and the Chinese National Standard method. This method was suitable for the rapid determination of B[a]P in mainstream cigarette smoke with the advantages of convenience, lower organic solvent consumption and higher sensitivity.关键词
卷烟/主流烟气/气相色谱-串联质谱/基质匹配溶剂/苯并[a]芘(B[a]P)Key words
Cigarette/Mainstream cigarette smoke/GC-MS/MS/Matrix matched solvent/Benzo[a]pyrene (B[a]P)分类
轻工纺织引用本文复制引用
廖惠云,王瑞,张华,吴洋,曹毅,张媛,王晨辉..基质匹配溶剂校准-GC-MS/MS法测定卷烟主流烟气B[a]P[J].烟草科技,2019,52(9):55-63,9.基金项目
江苏中烟工业有限责任公司科技项目"细支卷烟用爆珠对逐口烟气中主要香味物质释放量影响研究"(201703)、"细支卷烟中香精料液代表性组分释放转移分析及应用研究"(201704). （201703）
