广西医科大学学报2019，Vol.36Issue(10)：1549-1554,6.DOI:10.16190/j.cnki.45-1211/r.2019.10.001
利拉鲁肽和有氧运动对肥胖大鼠下丘脑弓状核神经元凋亡的影响
Effect of liraglutide and aerobic exercise on apoptosis of arcuate nucleus neurons in obese rats
摘要
Abstract
Objective:To investigate the effects of liraglutide and aerobic exercise on morphology and apoptosis of arcuate nucleus (ARC)neurons in obese rats.Methods:Obese rats were established by a high-fat diet and randomly divided into high-fat diet+saline (HS) group,high-fat diet+liraglutide (HL) group,and high-fat diet + aerobic exercise (HE) group.Untreated healthy rats served as the normal control(NC) group.The HL group rats received subcutaneous injection of liraglutide 200 μg/(kg· d).The NC group and HS group rats were given subcutaneous injection of the same volume of normal saline.Rats in the HE group were subjected to weightless swimming training for 60 min once day.After 6 weeks of intervention,the morphological structure of neurons in ARC of hypothalamus was observed by Nissl staining.The expression levels of Bax and Bcl-2 were determined by immunofluorescence.The apoptotic rate was detected by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining.Results:Compared with the NC group,ARC neurons were occasionally pyknotic and decreased in number in the hippocampus of the HS rats,and exhibited disordered arrangement,while milder neuronal injury and more cells were observed in the rats of the HL group and HE group.Compared with the HS group,liraglutide treatment or aerobic exercise decreased the number of TUNEL-positive cells and the expression of Bax concomitantly with upregulated Bcl-2 (P＜0.01).The changes were more marked in the HL group.Conclusion:Liraglutide could alleviate neuronal damages of the ARC by inhibiting apoptosis in obese rats,and its effect was superior to that of aerobic exercise.关键词
利拉鲁肽/有氧运动/肥胖大鼠/下丘脑/细胞凋亡Key words
liraglutide/aerobic exercise/obese rats/hypothalamus/cell apoptosis分类
医药卫生引用本文复制引用
杨威,赵胜男,荣曦,刘红..利拉鲁肽和有氧运动对肥胖大鼠下丘脑弓状核神经元凋亡的影响[J].广西医科大学学报,2019,36(10):1549-1554,6.基金项目
This study was supported by grants from the National Natural Science Foundation of China(No.81760154 （）
No.31600677)and Guangxi Natural Science Foundation (No.2017JJA10168). （No.2017JJA10168）
