中风与神经疾病杂志2019，Vol.36Issue(10)：931-935,5.
VPS35对多巴胺受体D1降解的调控机制研究
Mechanistic study on the degradation of dopamine receptor D1 by VPS35
摘要
Abstract
Objective To study whether the vacuolar protein sorting-35 (VPS35) could effect the process of degra-dation of G protein coupled receptor dopamine receptor D1 (DRD1) and its possible mechanism. Methods Depend on the cycloheximide (CHX) can restrain the synthesis of the proteins of the organism effectively. We handle cells with CHX to study whether VPS35 effects the degradation of DRD1. Results VPS35 accelerates the degradation of DRD1 after over-ex-pressing it in HEK 293T cells. On the contrary,after knock-down the VPS35 level the rate of degradation of DRD1 was re-duced. In addition,the VPS35 could accelerate the degradation of DRD1 through the pathway of lysosome and ubiquitin pro-teasome when the effect through pathway of lysosome is more significant. Conclusion VPS35 can regulate the degradation of DRD1 effectively mainly through the pathway of lysosome.关键词
囊泡分拣蛋白35/ 多巴胺受体D1/ 降解/ 帕金森病Key words
Vacuolar protein sorting-35/ Dopamine receptor D1/ Degradation/ Parkinson’s disease分类
医药卫生引用本文复制引用
王琛,肖乃安,马琪林,詹奕红..VPS35对多巴胺受体D1降解的调控机制研究[J].中风与神经疾病杂志,2019,36(10):931-935,5.基金项目
厦门大学附属第一医院院内青年科研发展基金资助项目(XYY2016015) （XYY2016015）
厦门市科技重大专项:厦门市重大疾病急救技术研究及急救网络体专项-脑卒中综合诊疗技术研究及救治网络建设(3502Z20171005-20170801) （3502Z20171005-20170801）
