烟草科技2019，Vol.52Issue(10)：1-12,12.
烟草miRNA169基因家族及启动子的生物信息学分析
Bioinformatic analysis of miRNA169 gene family and their promoters
摘要
Abstract
miRNA169 is a key factor involved in early flowering at low temperatures. In order to understand the expression and regulation mechanism of miRNA169 gene family in tobacco at low temperatures and to breed new tobacco varieties with resistance to low temperatures and early flowering, tobacco miRNA169 data in miRBase were analyzed. The structure and function of miRNA169 and their promoters in tobacco were predicted. The results showed that 19 miRNA169 genes were identified in tobacco genomes. They were distributed on chr01, chr08 and chr09 chromosomes. miRNA169 was found to cluster together in one chromosome. Except for pre-miRNA169t, the other pre-miRNA169 had high homology. LTR elements, corresponding to ABA and JA and involved in low temperature stress, were found on all promoters of miRNA169 in tobacco. Therefore, it was speculated that the response process of miRNA169 to low temperature stress might be the result of the interaction of LTR, ABA and JA pathways in tobacco.关键词
烟草/基因家族/miRNA 169/生物信息学/低温胁迫/启动子Key words
Tobacco/ Gene family/ miRNA169/ Bioinformatics/ Low temperature stress/ Promoter分类
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蔡健宇,贾蒙骜,张孝廉,余婧,李勇,雷波,郭玉双..烟草miRNA169基因家族及启动子的生物信息学分析[J].烟草科技,2019,52(10):1-12,12.基金项目
中国烟草总公司基因组计划重大专项项目"烟草抗病毒新基因NtTCTP功能研究及K326和云烟87品种PVY和TVBMV病毒病抗性的定向改良"[110201801026(JY-03)] （JY-03）
中国烟草总公司贵州省公司项目"烟草低温早花早期应答因子的筛选与抗性材料的创制"(201501) （201501）
烟草行业品质与生态重点实验室项目"控制烤烟叶片氮素分配的分子机制研究"(110201603009). （110201603009）
