烟草科技2019，Vol.52Issue(10)：13-21,9.
超量表达NrCN烟草光合特征及烟叶化学成分分析
Photosynthetic characteristics and chemical compositions of transgenic tobacco with overexpressed NrCN
摘要
Abstract
In order to clarify the effects of TMV-resistance gene NrCN on photosynthesis and chemical components of transgenic tobacco, T2 transgenic tobacco plants containing Pubi.u4::NrCN in the conditions of artificial climate incubators and greenhouse were used as research samples to determine the differential NrCN expression level, chemical compositions and photosynthetic characteristics in transgenic plants. The results showed that transgenic line Q2 with the strongest TMV resistance had the highest NrCN expression level and obvious lesions in leaves. In addition, transgenic line Q1 was stronger. The leaf chemical results indicated that the contents of total alkaloid and total nitrogen in Q2 line before and after topping were significantly lower than those of Q1 and Q3 lines, also lower than the non-transgenic line (P<0.05). However, chloride ion accumulation in Q2 line was higher before and after topping. The soluble sugar content (14.82 mg·g-1) in non-transgenic line before topping was significantly (P<0.01) lower than that in Q1 (21.88 mg·g-1) and Q2 lines (16.85 mg·g-1), respectively. Introducing NrCN gene could change the photosynthetic indexes of transgenic tobacco to different extents. Under a low light intensity (1 000 μmol·m-2·s-1), the net photosynthetic rate (Pn), stomatal conductance (Gs) and transpiration rate (Tr) of Q1 line increased significantly. Therefore, Q1 line could be used as an ideal parent material for breeding new tobacco germplasms with TMV resistance.关键词
烟草/NrCN基因/化学成分/光合指标/净光合速率/TMVKey words
Nicotiana tabacum/ NrCN/ Chemical composition/ Photosynthetic index/ Net photosynthetic rate/ TMV分类
农业科技引用本文复制引用
秦利军,赵德刚..超量表达NrCN烟草光合特征及烟叶化学成分分析[J].烟草科技,2019,52(10):13-21,9.基金项目
贵州省科技计划项目"烟草钾离子通道蛋白及BR对植株抗非生物胁迫研究"(黔科合LH[2016]7449号) （黔科合LH[2016]7449号）
贵州大学引进人才项目"利用基因编辑技术创制抗除草剂烟草新种质研究"(贵大人基合字[2015]24号) （贵大人基合字[2015]24号）
中国烟草总公司贵州省公司科技项目"烟草NAD1基因克隆及功能研究"(201608). （201608）
