烟草科技2019，Vol.52Issue(10)：22-27,6.
烟草赤星病菌AT-毒素胁迫处理对种子萌发的影响及抗病性鉴定
Effects of stress treatment by AT-toxin from Alternaria alternata on tobacco seed germination and its resistance identification
摘要
Abstract
In order to establish an efficient and accurate method to identify the resistance of tobacco varieties against tobacco brown spot, four tobacco varieties with different resistance levels were used to study the effects of different concentrations of AT-toxin from Alternaria alternate on tobacco seed germination and seedling root growth after treating for 10, 15 and 20 d. The results showed that the protein and soluble sugar contents in AT-toxin solution were 80.52 μg/mL and 23.39 mg/mL, respectively. With the increase of AT-toxin concentration, the germination rate of tobacco seeds and the percentage of tobacco seedlings with roots’growing into AT-toxin medium (PTS) decreased. Within the AT-toxin concentration and treatment time ranges, the tolerances of tobacco varieties to AT-toxin differed significantly. Comparing with G28, YN116 and Honghuadajinyuan, the PTS of Jingyehuang (resistant variety) were 1.18, 1.75, 6.02 times and 1.02, 1.75, 5.52 times in the 15th and 20th days after 50% AT-toxin treatment, respectively. Through the comprehensive analysis shown, PTS as an indicator of whether tobacco varieties resistant to brown spot is more accurate than seed germination rate, the optimal AT-toxin concentration is 50% and the optimal treatment time is 15-20 d.关键词
烟草品种/赤星病/AT-毒素/抗性鉴定Key words
Tobacco variety/ Brown spot/ AT-toxin/ Resistance identification分类
农业科技引用本文复制引用
卢小亮,张强,孙渭,陈鑫,吴洪启,陈耀锋..烟草赤星病菌AT-毒素胁迫处理对种子萌发的影响及抗病性鉴定[J].烟草科技,2019,52(10):22-27,6.基金项目
中国烟草总公司陕西省公司2016年科技计划项目"秦巴烟区优质烤烟新品种选育与区试"(KJ-2016-01). （KJ-2016-01）
