烟草科技2019，Vol.52Issue(10)：37-43,7.
在线热脱附-GC/MS法检测人体尿液中烟碱及可替宁
Quantifying nicotine and cotinine levels in human urine using automated thermal desorption-gas chromatography/mass spectrometry
汪阳忠 1李钢 1陶立奇 1吴达 1刘百战1
作者信息
- 1. 上海烟草集团有限责任公司技术中心,上海市杨浦区长阳路717号 200082
- 折叠
摘要
Abstract
In order to quantify the nicotine and cotinine levels in human urine, an automatic online thermal desorption-gas chromatography/mass spectrometry (GC/MS) method was developed. By using steam-treated SBA-15 type mesoporous silica as the sorbent and coupling with GC/MS, a device was designed for the efficient enrichment and rapid online analysis of nicotine and its metabolite cotinine in urine. The results showed that the device realized the online sample adsorption, thermal desorption, injection and analysis. The SBA-15 type mesoporous silica modified with steam at 700 ℃ for 6 h significantly improved the thermal desorption of nicotine and cotinine. Calibration curves in the range of 1-2 000 ng/mL of the analytes were established with R2 higher than 0.999 5. The spiked recoveries of the analytes ranged from 97.28% to 104.55% with the relative standard deviations(RSDs)of 0.25%-3.18%. The limits of detections for nicotine and cotinine were 0.5 and 0.4 ng/mL, respectively. This method is highly automatic, rapid and suitable for the analysis of nicotine and cotinine in human urine samples.关键词
吸烟者/尿液/烟碱/可替宁/GC/MS/改性SBA-15/热脱附Key words
Smoker/ Urine/ Nicotine/ Cotinine/ GC/MS/ Modified SBA-15/ Thermal desorption分类
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汪阳忠,李钢,陶立奇,吴达,刘百战..在线热脱附-GC/MS法检测人体尿液中烟碱及可替宁[J].烟草科技,2019,52(10):37-43,7.
