寄生虫与医学昆虫学报2019，Vol.26Issue(3)：137-144,8.DOI:10. 3969/j.issn.1005-0507. 2019. 03. 001
旋毛虫感染经PD-1途径对BCG免疫小鼠细胞因子产生的影响∗
TRICHINELLA SPIRALIS INFECTION IMPACTS ON THE BCG-INDUCED CYTOKINE PRODUCTION IN MICE VIA PD-1 PATHWAY
摘要
Abstract
To explore the role of PD-1 in effects of Trichinella spiralis infection on Mycobacterium bovis bacille Calmette-Guérin (BCG) -induced cytokine production in mice. First, wild-type (WT) and PD-1 deficient (PD-1-/-) mice bred on the C57BL/6 background were each infected with 400 infective T. spiralis muscle larvae and sacrificed at day 42 post infection. The splenocytes were isolated and cultured with stimulation of concanavalin A (ConA). The levels of Th1 cytokine IFN-γ, IL-2, TNF-α, Th2 cytokine IL-4 and regulatory cytokine TGF-β, IL-10 in the supernatants were measured by ELISA. Further, mice were immunized with BCG at day 28 post T. spiralis infection. The splenocytes collected respectively at day 7 and day 28 BCG immunization with or without T. spiralis infection were stimulated with BCG purified protein derivative (PPD). The levels of cytokines in the supernatants were measured by ELISA. The results showed that the tendencies of cytokine alteration were consistent in the two studies. In WT mice, the levels of Th1 cytokine IFN-γ, IL-2 in mice infected with T. spiralis were significantly lower than that of non-infected mice. The levels of Th2 cytokine IL-4 and regulatory cytokine TGF-β, IL-10 in infected mice were significantly higher than non-infected mice ( P<0. 05). No difference of the level of TNF-α was found between mice with or without T. spiralis infection. In PD-1-/- mice, the differences of decreased IFN-γ, IL-2 and increased TGF-β, IL-10 and IL-4 in infected mice were diminished compared with that in WT mice. The results demonstrate that T. spiralis infection may suppress the BCG-induced Th1 cytokine, but enhance Th2 and regulatory cytokine production through PD-1 pathway. This study provides experimental evidence for the potential mechanism that helminth infection suppresses the protective efficacy of BCG vaccination against tuberculosis.关键词
旋毛虫/ 程序性死亡分子1/ 卡介苗/ 细胞因子Key words
Trichinella spiralis/ PD-1/ BCG/ Cytokines引用本文复制引用
黄京京,呼延旭,庄清慧,康宁,杜素琴,程喻力,诸欣平..旋毛虫感染经PD-1途径对BCG免疫小鼠细胞因子产生的影响∗[J].寄生虫与医学昆虫学报,2019,26(3):137-144,8.基金项目
基金项目: 国家自然科学基金项目 (81572016) （81572016）
国家自然科学基金项目 (81672042) （81672042）
