数字中医药（英文）2018，Vol.1Issue(2)：115-121,7.
密蒙花颗粒对去势雄兔泪腺细胞凋亡因子Bax、Bcl-2、Fas和FasL的影响
Effect of Flos Buddlejae Granules on Apoptosis Factors Bax, Bcl-2, Fas and FasL in Lacrimal Gland Cells of Castrated Male Rabbits
摘要
Abstract
Objective To observe the effect of different concentration Flos Buddlejae (Mi Meng Hua, 密 蒙 花 ) granules on apoptosis factors of lacrimal gland cells of castrated male rabbits, and study the treatment effects of Flos Buddlejae (Mi Meng Hua, 密蒙花) granules in the dry eye model of castrated male rabbits. Methods Flos Buddlejae (Mi Meng Hua, 密 蒙 花 ) raw material was made into granules. Thirty healthy adult New Zealand rabbits were randomly divided into five groups, six rabbits in each group. Group A: blank group, Group B: model group, Group C: Flos Buddlejae (Mi Meng Hua, 密蒙花) granule group, Group D: placebo group, Group E: testosterone group. Except for group A, all rabbits underwent removal of bilateral testis and epididymis. Rabbits in group C were administered Flos Buddlejae (Mi Meng Hua, 密 蒙 花 ) granules (100 mg/kg), three times per day; rabbits in group D were administered normal saline, three times per day. Rabbits in group E were injected with testosterone propionate (0.5 mL/kg) in the thigh muscle, every 3 days. All rabbits were tested by Schirmer I test (SIT) and tear film break-up time (BUT) before operation and 4 weeks after operation. After 4 weeks, all rabbits were sacrificed by air embolism and clipping of the lacrimal gland. Apoptosis factors, including Bax, Bcl-2, Fas and FasL of lacrimal gland cells were characterized by immunohistochemistry, and resulting data were statistically analyzed. Results (1) Comparison of SIT and BUT before and after operation: There were statistically significant differences between groups B and D (P<0.01), but not among other groups (P>0.05). (2) Comparison of apoptosis factors Bax, Bcl-2 Fas and FasL: In a comparison of groups B and D, there was no statistically significant difference after operation(P>0.05). In a comparison of the other groups, there were statistically significant differences (P<0.01). In comparisons among A, C and E groups, there were no statistically significant differences (P>0.05). Conclusion Compared with androgen, Flos Buddlejae (Mi Meng Hua, 密蒙花) granules caused similar but slightly weaker depression of Bax, Fas and FasL, and increased expression of Bcl-2.关键词
密蒙花颗粒/干眼症/去势雄兔/泪腺细胞/细胞凋亡因子Key words
Flos Buddlejae Granules/Dry eye/Castrated male rabbits/Lacrimal gland cell/Apoptosis factors引用本文复制引用
欧阳云,彭俊,谭涵宇,李文娟,吴权龙,彭清华..密蒙花颗粒对去势雄兔泪腺细胞凋亡因子Bax、Bcl-2、Fas和FasL的影响[J].数字中医药（英文）,2018,1(2):115-121,7.基金项目
We thank for the funding support from the National Natural Science Foundation of China (NSFC) (No. 30772824 and No. 81574031) （NSFC）
225 Project of High-Level Medical Talents of Hunan Province, Research Project of Science and Technology Department of Hunan Province (No. 2015SF2016-6) （No. 2015SF2016-6）
Research Project of Hunan Provincial Development and Reform Commission (No. [2014]658) （No. [2014]658）
Major Project of Changsha Science and Technology Plan (K1501014-31) （K1501014-31）
Construction Project of Key Discipline of Chinese Ophthalmology of State Administration of Traditional Chinese Medicine （）
Construction Project of Key Discipline of Ophthalmology and Otolaryngology of Chinese Medicine of Hunan Province. （）
