数字中医药（英文）2018，Vol.1Issue(2)：164-172,9.
龙胆泻肝配方颗粒对复发型生殖器疱疹雌性豚鼠 炎症因子表达的影响
Long Dan Xie Gan Formula Granule Promotes Pro-Inflammatory Cytokine Expression in Female Guinea Pigs with Recurrent Genital Herpes
摘要
Abstract
Objective To explore the effects of Long Dan Xie Gan formula granule (LDXGFG) on regulation of pro-inflammatory cytokines in female guinea pigs with recurrent genital herpes (herpes simplex virus 2, HSV-2). Methods Levels of pro-inflammatory cytokines in blood of HSV-2-infected guinea pigs, including IL-6, IL-8, IL-10, IL-12, IFN-α, IFN-β, IFN-γ, and TNF-α, were detected by ELISA; corresponding gene expression levels in tissues were detected by real-time PCR. Results IL-6, IL-10, IL-12, IFN-α, IFN-γ and TNF-αdecreased significantly in both blood and diseased tissues after infection with recurrent genital herpes. Upon feeding LDXGFG to HSV-2-infected guinea pigs, IL-6, IL-10, IL-12, IFN-α, IFN-γ and TNF-α demonstrated significant increases, similar to the effects of acyclovir (ACV). LDXGFG promoted the expression of pro-inflammatory cytokines in blood and tissue, with a stronger effect than ACV. Moreover, LDXGFG demonstrated broader effects than ACV. Conclusion The present results suggest that LDXGFG can serve as an alternative, inexpensive, and long-term treatment for HSV-2 infection.关键词
复发性生殖器疱疹/单纯疱疹病毒Ⅱ型/龙胆泻肝配方颗粒/炎症因子/阿昔洛韦/豚鼠Key words
Recurrent genital herpes/HSV-2/Long Dan Xie Gan formula granule (LDXGFG)/Pro-inflammatory cytokines/ACV/Guinea pig引用本文复制引用
匡琳,黄恩惠,何清湖,成绍武,刘晓丹..龙胆泻肝配方颗粒对复发型生殖器疱疹雌性豚鼠 炎症因子表达的影响[J].数字中医药（英文）,2018,1(2):164-172,9.基金项目
We thank for the funding support from Chinese Nature Science Foundation (No. 81202705), The Effect of Long Dan Xie Gan Formula Granule on Toll Signaling Pathway in the Guinea pigs of Recurrent Genital Herpes Changsha Science and Technology Foundation (No. kh1601195) （No. 81202705）
