数字中医药（英文）2018，Vol.1Issue(3)：219-227,9.
慢性束缚大鼠海马差异蛋白质表达及逍遥散干预作用
Differentially Expressed Proteins in Rat Hippocampus after Chronic Immobilization Stress and Intervention Using Xiao Yao San Decoction
摘要
Abstract
Objective To identify differentially expressed proteins in the hippocampus of rats after chronic immobilization stress (CIS) using a proteomics approach, and to study the effect of the Xiao Yao San (XYS) decoction on differentially expressed proteins. Methods Twenty-four Sprague Dawley rats were randomly assigned to one of four groups of equal body weight: control (non-stress), 7-day stress, 21-day stress and 21-day stress +XYS treatment groups. Two-dimensional gel electrophoresis (2-DE) was used to detect differences in protein expression in rat hippocampus. One differentially expressed protein was measured and verified by western blotting. Results Seventeen proteins showed differential expression. Among these, eight could be identified: glial fibrillary acidic protein-2 (GFAP-2), tubulin alpha-1c, cytoplasmic muscle actin 2, 14-3-3 protein, β-2a tubulin, phosphatidylethanolamine binding protein, synuclein α syn3, and a low molecular weight (18 kD) protein. Six of these proteins exhibited increased expression, one showed decreased expression, and the other protein, which comprised five subtypes, were either increased or decreased. These proteins are known to be involved in immunity, signal transduction, cell cycle control, apoptosis, regulation of enzyme activity, cytoskeleton structure, and synaptic plasticity. GFAP-2 was further analyzed, and its differential expression confirmed by western blotting. Conclusion Some proteins are differentially expressed in the hippocampus of rats under chronic stress. The biological functions of these differentially expressed proteins are varied. Finally, the XYS decoction can significantly up- or down-regulate these protein expression levels.关键词
慢性束缚应激/肝郁脾虚证/大鼠海马/逍遥散/差异表达蛋白质Key words
Chronic immobilization stress/Syndrome of liver-depression and spleen-deficiency/Rat Hippocampus/XYS decoction/Differentially expressed proteins引用本文复制引用
寇美静,薛哲,刘雁云,刘玥芸,刘燕,陈家旭..慢性束缚大鼠海马差异蛋白质表达及逍遥散干预作用[J].数字中医药（英文）,2018,1(3):219-227,9.基金项目
We thank for the funding support from the National Natural Science Foundation of China (NO. 81473597), China National Funds for Distinguished Young Scientists (NO. 30825046), and supported by the Beijing Natural Sciences Foundation (NO. 7152093). （NO. 81473597）
